ArticleACS synthetic biology2014
Rule-based design of synthetic transcription factors in eukaryotes.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 0 citations in OpenAlex.
- Cross-Activity Analysis of CRISPR/Cas9 Editing in Gene Families ofCurrent issues in molecular biology · 2025Article
- The art of designed coiled-coils for the regulation of mammalian cells.Cell chemical biology · 2024Review
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Programmable T7-based synthetic transcription factors.Nucleic acids research · 2018Article
- Discovery of cell-type specific DNA motif grammar in cis-regulatory elements using random Forest.BMC genomics · 2018Article
- A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy.ACS synthetic biology · 2015Article
- Engineering protocells: prospects for self-assembly and nanoscale production-lines.Life (Basel, Switzerland) · 2015Review
- Rule-Based Design of Plant Expression Vectors Using GenoCAD.PloS one · 2015Article
- Transcription activator-like effectors: a toolkit for synthetic biology.ACS synthetic biology · 2014Review
- Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.Molecular cell · 2014Article
- Principles of genetic circuit design.Nature methods · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
To design and build living systems, synthetic biologists have at their disposal an increasingly large library of naturally derived and synthetic parts. These parts must be combined together in particular orders, orientations, and spacings to achieve desired functionalities. These structural constraints can be viewed as grammatical rules describing how to assemble parts together into larger functional units. Here, we develop a grammar for the design of synthetic transcription factors (sTFs) in eukaryotic cells and implement it within GenoCAD, a Computer-Aided Design (CAD) software for synthetic biology. Knowledge derived from experimental evidence was captured in this grammar to guide the user to create designer transcription factors that should operate as intended. The grammar can be easily updated and refined as our experience with using sTFs in different contexts increases. In combination with grammars that define other synthetic systems, we anticipate that this work will enable the more reliable, efficient, and automated design of synthetic cells with rich functionalities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.