ArticlePloS one2014
Tracking endogenous amelogenin and ameloblastin in vivo.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- A novelFrontiers in physiology · 2026Article
- Skeletal site-specific variations in myeloid cells: insights from single-cell RNA sequencing of the mandible and femur.JBMR plus · 2025Article
- Expression ofInternational journal of molecular sciences · 2025Article
- Ameloblastin binding to biomimetic models of cell membranes - A continuum of intrinsic disorder.Archives of oral biology · 2025Article
- Ameloblastin and its multifunctionality in amelogenesis: A review.Matrix biology : journal of the International Society for Matrix Biology · 2024Review
- The Dynamic Interactions of a Multitargeting Domain in Ameloblastin Protein with Amelogenin and Membrane.International journal of molecular sciences · 2023Article
- Early evolution of enamel matrix proteins is reflected by pleiotropy of physiological functions.Scientific reports · 2023Article
- The Role of Type VI Collagen in Alveolar Bone.International journal of molecular sciences · 2022Article
- An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch.The Journal of biological chemistry · 2022Article
- Altered morpho-functional features of bones in autoimmune disease-prone BXSB/MpJ- Yaa mice.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns.Frontiers in physiology · 2019Article
- The importance of a potential phosphorylation site in enamelin on enamel formation.International journal of oral science · 2017Article
- Role of MIZ-1 inBiochemistry and biophysics reports · 2016Article
- Expression of amelogenin and effects of cyclosporin A in developing hair follicles in rats.Journal of anatomy · 2016Article
- Amelogenins as potential buffers during secretory-stage amelogenesis.Journal of dental research · 2015Article
- Comparison of two mouse ameloblast-like cell lines for enamel-specific gene expression.Frontiers in physiology · 2014Article
- MSX2 in ameloblast cell fate and activity.Frontiers in physiology · 2014Review
- Novel biological activity of ameloblastin in enamel matrix derivative.Journal of applied oral science : revista FOBArticle
Corrections and comments
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Authors and funding
10 authors at 9 institutions in 4 countries.
Funding
Abstract
Research on enamel matrix proteins (EMPs) is centered on understanding their role in enamel biomineralization and their bioactivity for tissue engineering. While therapeutic application of EMPs has been widely documented, their expression and biological function in non-enamel tissues is unclear. Our first aim was to screen for amelogenin (AMELX) and ameloblastin (AMBN) gene expression in mandibular bones and soft tissues isolated from adult mice (15 weeks old). Using RT-PCR, we showed mRNA expression of AMELX and AMBN in mandibular alveolar and basal bones and, at low levels, in several soft tissues; eyes and ovaries were RNA-positive for AMELX and eyes, tongues and testicles for AMBN. Moreover, in mandibular tissues AMELX and AMBN mRNA levels varied according to two parameters: 1) ontogenic stage (decreasing with age), and 2) tissue-type (e.g. higher level in dental epithelial cells and alveolar bone when compared to basal bone and dental mesenchymal cells in 1 week old mice). In situ hybridization and immunohistodetection were performed in mandibular tissues using AMELX KO mice as controls. We identified AMELX-producing (RNA-positive) cells lining the adjacent alveolar bone and AMBN and AMELX proteins in the microenvironment surrounding EMPs-producing cells. Western blotting of proteins extracted by non-dissociative means revealed that AMELX and AMBN are not exclusive to mineralized matrix; they are present to some degree in a solubilized state in mandibular bone and presumably have some capacity to diffuse. Our data support the notion that AMELX and AMBN may function as growth factor-like molecules solubilized in the aqueous microenvironment. In jaws, they might play some role in bone physiology through autocrine/paracrine pathways, particularly during development and stress-induced remodeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.