ArticlePloS one2014
Label-free determination of hemodynamic parameters in the microcirculaton with third harmonic generation microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- ECgo: All-optical induction of single endothelial cell injury and capillary occlusion in the brain.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Live imaging of vascular dynamics in mouse skin.The Journal of investigative dermatology · 2026Article
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- Label-free, whole-brain in vivo mapping in an adult vertebrate with third harmonic generation microscopy.The Journal of comparative neurology · 2024Article
- Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning.Biomedical optics express · 2022Article
- Progressive stretch enhances growth and maturation of 3D stem-cell-derived myocardium.Theranostics · 2021Article
- Label-free assessment of hemodynamics in individual cortical brain vessels using third harmonic generation microscopy.Biomedical optics express · 2020Article
- In vivo deep-brain blood flow speed measurement through third-harmonic generation imaging excited at the 1700-nm window.Biomedical optics express · 2020Article
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Miniaturized multimodal multiphoton microscope for simultaneous two-photon and three-photon imaging with a dual-wavelength Er-doped fiber laser.Biomedical optics express · 2020Article
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- Leukocyte Trafficking and Hemostasis in the Mouse FetusFrontiers in cell and developmental biology · 2020Article
- Surgical preparations, labeling strategies, and optical techniques for cell-resolved, in vivo imaging in the mouse spinal cord.Experimental neurology · 2019Review
- Unraveling the host's immune response to infection: Seeing is believing.Journal of leukocyte biology · 2019Review
- Spatiotemporally controlled nano-sized third harmonic generation agents.Biomedical optics express · 2019Article
- Second harmonic generation microscopy of early embryonic mouse hearts.Biomedical optics express · 2019Article
- Estimating hemodynamic shear stress in murine peripheral collateral arteries by two-photon line scanning.Molecular and cellular biochemistry · 2019Article
- Apoε4 disrupts neurovascular regulation and undermines white matter integrity and cognitive function.Nature communications · 2018Article
- Cancer Immunotherapy Getting Brainy: Visualizing the Distinctive CNS Metastatic Niche to Illuminate Therapeutic Resistance.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2017Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of blood flow velocity and related hemodynamic parameters is an important aspect of physiological studies which in many settings requires fluorescent labeling. Here we show that Third Harmonic Generation (THG) microscopy is a suitable tool for label-free intravital investigations of the microcirculation in widely-used physiological model systems. THG microscopy is a non-fluorescent multi-photon scanning technique combining the advantages of label-free imaging with restriction of signal generation to a focal spot. Blood flow was visualized and its velocity was measured in adult mouse cremaster muscle vessels, non-invasively in mouse ear vessels and in Xenopus tadpoles. In arterioles, THG line scanning allowed determination of the flow pulse velocity curve and hence the heart rate. By relocating the scan line we obtained velocity profiles through vessel diameters, allowing shear rate calculations. The cell free layer containing the glycocalyx was also visualized. Comparison of the current microscopic resolution with theoretical, diffraction limited resolution let us conclude that an about sixty-fold THG signal intensity increase may be possible with future improved optics, optimized for 1200-1300 nm excitation. THG microscopy is compatible with simultaneous two-photon excited fluorescence detection. It thus also provides the opportunity to determine important hemodynamic parameters in parallel to common fluorescent observations without additional label.
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