ArticleTechnology2014
A novel ultrathin collagen nanolayer assembly for 3-D microtissue engineering: Layer-by-layer collagen deposition for long-term stable microfluidic hepatocyte culture.
Article in Technology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Protein-based layer-by-layer films for biomedical applications.Chemical science · 2024Review
- Evaluation of drug carrier hepatotoxicity using primary cell culture models.Nanomedicine : nanotechnology, biology, and medicine · 2023Article
- Physiologically relevant microsystems to study viral infection in the human liver.Frontiers in microbiology · 2022Review
- Progressive hypoxia-on-a-chip: An in vitro oxygen gradient model for capturing the effects of hypoxia on primary hepatocytes in health and disease.Biotechnology and bioengineering · 2020Article
- Article
- A microfluidic 3D hepatocyte chip for hepatotoxicity testing of nanoparticles.Nanomedicine (London, England) · 2019Article
- Metabolic Patterning on a Chip: Towards in vitro Liver Zonation of Primary Rat and Human Hepatocytes.Scientific reports · 2018Article
- Dose-, treatment- and time-dependent toxicity of superparamagnetic iron oxide nanoparticles on primary rat hepatocytes.Nanomedicine (London, England) · 2018Article
- Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy.Engineering in life sciences · 2017Article
- Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology.Technology · 2017Article
- A Microfabricated Platform for Generating Physiologically-Relevant Hepatocyte Zonation.Scientific reports · 2016Article
- Long-term maintenance of a microfluidic 3D human liver sinusoid.Biotechnology and bioengineering · 2016Article
- Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids.Stem cell reports · 2015Article
- Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization.Journal of visualized experiments : JoVE · 2015Article
- Biotunable acoustic node assembly of organoids.Advanced healthcare materials · 2015Article
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
- Regulation and disregulation of mammalian nucleotide excision repair: a pathway to nongermline breast carcinogenesis.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The creation of stable hepatocyte cultures using cell-matrix interactions has proven difficult in microdevices due to dimensional constraints limiting the utility of classic tissue culture techniques that involve the use of hydrogels such as the collagen "double gel" or "overlay". To translate the collagen overlay technique into microdevices, we modified collagen using succinylation and methylation reactions to create polyanionic and polycationic collagen solutions, and deposited them layer-by-layer to create ultrathin collagen nanolayers on hepatocytes. These ultrathin collagen layers covered hepatocytes in microdevices and 1) maintained cell morphology, viability, and polarity, 2) induced bile canalicular formation and actin reorganization, and 3) maintained albumin and urea secretions and CYP activity similar to those observed in hepatocytes in collagen double gel hepatocytes in plate cultures. Beyond the immediate applications of this technique to create stable,
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.