ArticleMuscles, ligaments and tendons journal2014
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment.
Article in Muscles, ligaments and tendons journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Chinese medicine boosts regenerative medicine in stem cell - based therapy.Stem cell research & therapy · 2025Review
- Fibrin Scaffolds Perfused with Transforming Growth Factor-β1 as an In Vitro Model to Study Healthy and Tendinopathic Human Tendon Stem/Progenitor Cells.International journal of molecular sciences · 2024Article
- Biological approaches to the repair and regeneration of the rotator cuff tendon-bone enthesis: a literature review.Biomaterials translational · 2023Review
- Natural, synthetic and commercially-available biopolymers used to regenerate tendons and ligaments.Bioactive materials · 2023Review
- Basic Research on Tendon Repair: Strategies, Evaluation, and Development.Frontiers in medicine · 2021Review
- A brief history of tendon and ligament bioreactors: Impact and future prospects.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits.Stem cells international · 2020Article
- Use of stem cells and growth factors in rotator cuff tendon repair.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2019Review
- Combined ascorbic acid and TJournal of orthopaedic surgery and research · 2019Article
- Human Subacromial Bursal Cells Display Superior Engraftment Versus Bone Marrow Stromal Cells in Murine Tendon Repair.The American journal of sports medicine · 2018Article
- Regenerative biology of tendon: mechanisms for renewal and repair.Current molecular biology reports · 2015Article
- Three dimensional de novo micro bone marrow and its versatile application in drug screening and regenerative medicine.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Stem cell technology for tendon regeneration: current status, challenges, and future research directions.Stem cells and cloning : advances and applications · 2015Review
- Advanced strategies for constructing interfacial tissues of bone and tendon/ligament.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductioncell-based tissue engineering strategies using human mesenchymal stem cells (hMSCs) may help to augment tendon healing. To further investigate the in-vitro behavior of this cell population, we investigated low oxygen culture levels, and growth factor supplementation and their effect on expression of tendon extracellular proteins and cell proliferation. MATERIALS AND
methodsbone marrow aspirate (BMA) was harvested during arthroscopic rotator cuff repair. Characterized hMSCs derived from BMA were incorporated into 3-dimensional tissue engineered constructs (TECs). TECs were analyzed by frozen sections with immunohistochemistry for cell density, collagen I and collagen III expression.
resultsgrowth factor stimulation and low oxygen increased cell density within TECs. Low oxygen and addition of growth factors to culture media demonstrated an increase in collagen I and III expression, both in ambient oxygen conditions and low oxygen conditions.
conclusionlow oxygen and TGFβ3 demonstrated a positive effect on cell number, and type I and III collagen expression in 3D culture environments.
Indexed as
Identifiers
24932447PMC4049650W1498392854What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.