ArticlePlant methods2014
A virus-induced gene silencing (VIGS) system for functional genomics in the parasitic plant Striga hermonthica.
Article in Plant methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Genome-Wide Analysis of thePlants (Basel, Switzerland) · 2026Article
- Genome-wide analysis of theFrontiers in plant science · 2026Article
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- RNAi technology development for weed control: all smoke and no fire?Pest management science · 2025Review
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Establishing a Virus-Induced Gene Silencing System inPlants (Basel, Switzerland) · 2023Article
- Molecular actors of seed germination and haustoriogenesis in parasitic weeds.Plant physiology · 2021Article
- Review
- Breeding maize (Plant breeding = Zeitschrift fur Pflanzenzuchtung · 2021Review
- Generation of Transgenic Rice Expressing CircRNA and Its Functional Characterization.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Effect of Virulence and Resistance Mechanisms on the Interactions between Parasitic Plants and Their Hosts.International journal of molecular sciences · 2020Review
- Plant science's next top models.Annals of botany · 2020Review
- Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize.Frontiers in plant science · 2017Article
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStriga hermonthica is a hemiparasitic weed that infects cereals in Sub Sahara Africa (SSA) resulting in up to 100% grain yield loss. This significant loss in grain yields is a major contributor to food insecurity and poverty in the region. Current strategies to control the parasite are costly, unavailable and remain unpracticed by small-scale farmers, underscoring the need for more economical and sustainable control strategies. Development of resistant germplasm is the most sustainable strategy in the control of S. hermonthica, but is constrained by paucity of resistance genes for introduction into crop germplasm. RNA interference (RNAi) has potential for developing host-derived resistance against S. hermonthica by transformation of host crops with RNAi sequences targeted at critical Striga genes. The application of RNAi in management of S. hermonthica is however constrained by lack of efficient high throughput screening protocols for the candidate genes for silencing, as well as sub optimal delivery of siRNAs into the parasite. In comparison to stable transformation, viral induced gene silencing (VIGS) is a rapid and powerful tool for plant functional genomics and provides an easy and effective strategy in screening for putative candidate genes to target through RNAi. In addition, VIGS allows for a secondary amplification of the RNAi signal increasing the siRNA threshold and facilitates siRNA transport through viral movement proteins. We tested the efficiency of the Tobacco rattle virus (TRV1 and TRV2) VIGS vectors in silencing S. hermonthica phytoene desaturase (PDS) gene through agrodrench and agro-infiltration.
resultsWe report the validation of VIGS in S. hermonthica using a silencing cassette generated from TRV with a PDS gene insert. Agro-infiltrated and agro-drenched S. hermonthica leaves showed photo-bleaching phenotypes typical for PDS silencing within 7 and 14 days post infection respectively. In both cases S. hermonthica plants recovered from photo-bleaching effects within 28 days post inoculation. The transformation efficiency of the VIGS protocol in S. hermonthica was (60 ± 2.9)%.
conclusionThese results demonstrate that the TRV-VIGS system work in S. hermonthica and can be used for candidate gene validation for their role in the parasite development and parasitism, with the ultimate goal of developing resistant transgenic maize.
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