ArticleCell reports2014
A conditional system to specifically link disruption of protein-coding function with reporter expression in mice.
Article in Cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Hmga2-dependent cAMP signaling drives UVB-induced melanocyte stem cell activation.The Journal of investigative dermatology · 2026Article
- Conditional replacement of the mouse luteinizing hormone receptor with green fluorescent protein, enabling imaging of cell migration during ovulation.Endocrinology · 2026Article
- Conditional replacement of the mouse LH receptor with GFP, enabling imaging of cell migration during ovulation.bioRxiv : the preprint server for biology · 2026Article
- Combinatorial In Vivo Genome Editing Identifies Widespread Epistasis and an Accessible Fitness Landscape During Lung Tumorigenesis.Molecular biology and evolution · 2025Article
- Article
- HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation.Nature communications · 2023Article
- Hmga2 deficiency is associated with allometric growth retardation, infertility, and behavioral abnormalities in mice.G3 (Bethesda, Md.) · 2022Article
- Genetic Determinants of EGFR-Driven Lung Cancer Growth and Therapeutic ResponseCancer discovery · 2021Article
- Hmga2 is dispensable for pancreatic cancer development, metastasis, and therapy resistance.Scientific reports · 2018Article
- BLIMP1 Induces Transient Metastatic Heterogeneity in Pancreatic Cancer.Cancer discovery · 2017Article
- Recombinase-based conditional and reversible gene regulation via XTR alleles.Nature communications · 2015Article
- Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Conditional gene deletion in mice has contributed immensely to our understanding of many biological and biomedical processes. Despite an increasing awareness of nonprotein-coding functional elements within protein-coding transcripts, current gene-targeting approaches typically involve simultaneous ablation of noncoding elements within targeted protein-coding genes. The potential for protein-coding genes to have additional noncoding functions necessitates the development of novel genetic tools capable of precisely interrogating individual functional elements. We present a strategy that couples Cre/loxP-mediated conditional gene disruption with faithful GFP reporter expression in mice in which Cre-mediated stable inversion of a splice acceptor-GFP-splice donor cassette concurrently disrupts protein production and creates a GFP fusion product. Importantly, cassette inversion maintains physiologic transcript structure, thereby ensuring proper microRNA-mediated regulation of the GFP reporter, as well as maintaining expression of nonprotein-coding elements. To test this potentially generalizable strategy, we generated and analyzed mice with this conditional knockin reporter targeted to the Hmga2 locus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.