ArticleCell reports2014
A rapid genome-wide microRNA screen identifies miR-14 as a modulator of Hedgehog signaling.
Article in Cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
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- Distinct small RNAs are expressed at different stages ofFrontiers in genetics · 2024Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inbioRxiv : the preprint server for biology · 2023Article
- Photoreceptors generate neuronal diversity in their target field through a Hedgehog morphogen gradient ineLife · 2022Article
- Small ORFs as New Regulators of Pri-miRNAs and miRNAs Expression inInternational journal of molecular sciences · 2022Article
- Dissection of the microRNA Network Regulating Hedgehog Signaling inFrontiers in cell and developmental biology · 2022Article
- Nanoparticle-Based RNAi Therapeutics Targeting Cancer Stem Cells: Update and Prospective.Pharmaceutics · 2021Review
- The multifaceted roles of microRNAs in differentiation.Current opinion in cell biology · 2020Review
- Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Global identification of functional microRNA-mRNA interactions in Drosophila.Nature communications · 2019Article
- mir-124-5p Regulates Phagocytosis of Human Macrophages by Targeting the Actin Cytoskeleton via the ARP2/3 Complex.Frontiers in immunology · 2019Article
- Synaptojanin regulates Hedgehog signalling by modulating phosphatidylinositol 4-phosphate levels.Journal of biosciences · 2018Article
- Endocrine and physiological regulation of neutral fat storage in Drosophila.Molecular and cellular endocrinology · 2018Review
- MicroRNA function in Drosophila melanogaster.Seminars in cell & developmental biology · 2017Review
- Article
- TGF-β1-induced miR-202 mediates drug resistance by inhibiting apoptosis in human osteosarcoma.Journal of cancer research and clinical oncology · 2016Article
- Regulation of physiological processes by microRNAs in insects.Current opinion in insect science · 2015Article
- Overview of microRNA biology.Seminars in liver disease · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by binding to sequences within the 3' UTR of mRNAs. Because miRNAs bind to short sequences with partial complementarity, target identification is challenging. To complement the existing target prediction algorithms, we devised a systematic "reverse approach" screening platform that allows the empirical prediction of miRNA-target interactions. Using Drosophila cells, we screened the 3' untranslated regions (3' UTRs) of the Hedgehog pathway genes against a genome-wide miRNA library and identified both predicted and many nonpredicted miRNA-target interactions. We demonstrate that miR-14 is essential for maintaining the proper level of Hedgehog signaling activity by regulating its physiological target, hedgehog. Furthermore, elevated levels of miR-14 suppress Hedgehog signaling activity by cotargeting its apparent nonphysiological targets, patched and smoothened. Altogether, our systematic screening platform is a powerful approach to identifying both physiological and apparent nonphysiological targets of miRNAs, which are relevant in both normal and diseased tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.