ArticleMolecular microbiology2014
Differential affinities of MinD and MinE to anionic phospholipid influence Min patterning dynamics in vitro.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Fast, StreamlinedChemical & biomedical imaging · 2026Article
- Cell-Size Confinement Drives Size-Dependent Scaling of Intracellular Reaction-Diffusion Waves for Robust Patterning.Small science · 2026Article
- 3D pattern formation of a protein-membrane suspension.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Review
- Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division.Nature communications · 2024Article
- Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins.Communications biology · 2024Article
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- Forceful patterning: theoretical principles of mechanochemical pattern formation.EMBO reports · 2023Review
- The mechanism of MinD stability modulation by MinE in Min protein dynamics.PLoS computational biology · 2023Article
- Measuring flow-mediated protein drift across stationary supported lipid bilayers.Biophysical journal · 2023Article
- Directing Min protein patterns with advective bulk flow.Nature communications · 2023Article
- Bridging scales in a multiscale pattern-forming system.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells.Science advances · 2022Article
- Cardiolipin-Containing Lipid Membranes Attract the Bacterial Cell Division Protein DivIVA.International journal of molecular sciences · 2021Article
- Increasing MinD's Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes.ACS synthetic biology · 2021Article
- Engineering spatiotemporal organization and dynamics in synthetic cells.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021Review
- Cardiolipin aids in lipopolysaccharide transport to the gram-negative outer membrane.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles.Angewandte Chemie (International ed. in English) · 2021Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
The E. coli Min system forms a cell-pole-to-cell-pole oscillator that positions the divisome at mid-cell. The MinD ATPase binds the membrane and recruits the cell division inhibitor MinC. MinE interacts with and releases MinD (and MinC) from the membrane. The chase of MinD by MinE creates the in vivo oscillator that maintains a low level of the division inhibitor at mid-cell. In vitro reconstitution and visualization of Min proteins on a supported lipid bilayer has provided significant advances in understanding Min patterns in vivo. Here we studied the effects of flow, lipid composition, and salt concentration on Min patterning. Flow and no-flow conditions both supported Min protein patterns with somewhat different characteristics. Without flow, MinD and MinE formed spiraling waves. MinD and, to a greater extent MinE, have stronger affinities for anionic phospholipid. MinD-independent binding of MinE to anionic lipid resulted in slower and narrower waves. MinE binding to the bilayer was also more susceptible to changes in ionic strength than MinD. We find that modulating protein diffusion with flow, or membrane binding affinities with changes in lipid composition or salt concentration, can differentially affect the retention time of MinD and MinE, leading to spatiotemporal changes in Min patterning.
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