ArticleScientific reports2014
Extending the viability of acute brain slices.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Chemogenetic Activation of LC Noradrenergic Afferents Facilitates Cerebellar CF-PC LTD via Presynaptic αBiomolecules · 2026Article
- Human brain slice cultures: translational applications and ethical considerations.Life science alliance · 2025Review
- Enhanced Response of ZnO Nanorod-Based Flexible MEAs for Recording Ischemia-Induced Neural Activity in Acute Brain Slices.Nanomaterials (Basel, Switzerland) · 2025Article
- Potassium homeostasis during disease progression of Alzheimer's disease.The Journal of physiology · 2025Article
- Cellular morphodynamics as quantifiers for functional states of resident tissue macrophages in vivo.PLoS computational biology · 2025Article
- Recurrent activity propagates through labile ensembles in macaque dorsolateral prefrontal microcircuits.Current biology : CB · 2025Article
- Personalising glioblastoma medicine: explant organoid applications, challenges and future perspectives.Acta neuropathologica communications · 2025Review
- Enhanced electrophysiological recordings in acute brain slices, spheroids, and organoids using 3D high-density multielectrode arrays.PloS one · 2025Article
- Microbubble dynamics in brain microvessels.PloS one · 2025Article
- Recording of Age-Related Changes on Murine and Human Brain Slices.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Phosphodiesterase inhibition and Gucy2C activation enhance tyrosine hydroxylase Ser40 phosphorylation and improve 6-hydroxydopamine-induced motor deficits.Cell & bioscience · 2024Article
- A high-density multi-electrode platform examining the effects of radiation on in vitro cortical networks.Scientific reports · 2024Article
- Trauma in Neonatal Acute Brain Slices Alters Calcium and Network Dynamics and Causes Calpain-Mediated Cell Death.eNeuro · 2024Article
- Time-dependent phenotypical changes of microglia drive alterations in hippocampal synaptic transmission in acute slices.Frontiers in cellular neuroscience · 2024Article
- Chronic neuroinflammation during aging leads to cholinergic neurodegeneration in the mouse medial septum.Journal of neuroinflammation · 2023Article
- A living ex vivo platform for functional, personalized brain cancer diagnosis.Cell reports. Medicine · 2023Article
- Live Cell Light Sheet Imaging with Low- and High-Spatial-Coherence Detection Approaches Reveals Spatiotemporal Aspects of Neuronal Signaling.Journal of imaging · 2023Article
- Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice culture.STAR protocols · 2022Article
- Preparation of acute midbrain slices containing the superior colliculus and periaqueductal Gray for patch-clamp recordings.PloS one · 2022Article
- Three dimensional microelectrodes enable high signal and spatial resolution for neural seizure recordings in brain slices and freely behaving animals.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lifespan of an acute brain slice is approximately 6-12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.