Evidence map›Paper›PMID 24930852›Full record

ArticleBiomaterials2014

Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS.

Lan T H Dang, Nicole T Feric, Carol Laschinger, Wing Y Chang, Boyang Zhang, Geoffrey A Wood, William L Stanford, Milica Radisic

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
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  6. Review
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  9. Article
  10. Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  11. IGF-1 C Domain-Modified Hydrogel Enhances Cell Therapy for AKI.Journal of the American Society of Nephrology : JASN · 2016
    Article
  12. Stem cell death and survival in heart regeneration and repair.Apoptosis : an international journal on programmed cell death · 2016
    Review
  13. Review
  14. Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Lan T H DangInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada; The Heart and Stroke/Richard Lewar Centre of Excellence, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Nicole T FericInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada.
Carol LaschingerInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada.
Wing Y ChangInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada; Sprott Centre for Stem Cell Research, The Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, Canada; Department of Cellular & Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada.
Boyang ZhangDepartment of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada.
Geoffrey A WoodDepartment of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
William L StanfordInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada; Sprott Centre for Stem Cell Research, The Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, Canada; Department of Cellular & Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada.
Milica RadisicInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada; The Heart and Stroke/Richard Lewar Centre of Excellence, University of Toronto, Toronto, Ontario M5S 1A8, Canada; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada. Electronic address: m.radisic@utoronto.ca.
University of Toronto · CAOttawa Hospital Research InstituteUniversity of Guelph · CA

Funding

Engineering Vascularized Cardiac MuscleR01HL076485 · NHLBI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Gordana Vunjak-Novakovic · 2005 to 2026
$9.2M
CIHR 385981-10CIHR MOP-126027NHLBI NIH HHS 2R01 HL076485NHLBI NIH HHS R01 HL076485
6 · The paper itself

Abstract

Adhesion molecule signaling is critical to human pluripotent stem cell (hPSC) survival, self-renewal, and differentiation. Thus, hPSCs are grown as clumps of cells on feeder cell layers or poorly defined extracellular matrices such as Matrigel. We sought to define a small molecule that would initiate adhesion-based signaling to serve as a basis for a defined substrate for hPSC culture. Soluble angiopoeitin-1 (Ang-1)-derived peptide QHREDGS added to defined serum-free media increased hPSC colony cell number and size during long- and short-term culture when grown on feeder cell layers or Matrigel, i.e. on standard substrates, without affecting hPSC morphology, growth rate or the ability to differentiate into multiple lineages both in vitro and in vivo. Importantly, QHREDGS treatment decreased hPSC apoptosis during routine passaging and single-cell dissociation. Mechanistically, the interaction of QHREDGS with β1-integrins increased expression of integrin-linked kinase (ILK), increased expression and activation of extracellular signal-regulated kinases 1/2 (ERK1/2), and decreased caspase-3/7 activity. QHREDGS immobilization to polyethylene glycol hydrogels significantly increased cell adhesion in a dose-dependent manner. We propose QHREDGS as a small molecule inhibitor of hPSC apoptosis and the basis of an affordable defined substrate for hPSC maintenance.

Indexed as

Angiopoietin-1AnimalsApoptosisCaspasesCell AdhesionCell CountCell Culture TechniquesCell ProliferationCells, CulturedCell SizeCell SurvivalEnzyme ActivationExtracellular Signal-Regulated MAP KinasesFeeder CellsHumansInduced Pluripotent Stem CellsAngiopoietin-1CaspasesExtracellular Signal-Regulated MAP KinasesIntegrin beta1PeptidesProtein Serine-Threonine KinasesScaffold Protein ILKAdhesionAngiopoietin-1ApoptosisHuman pluripotent stem cellsIntegrinsQHREDGS

Identifiers

PMID24930852
PMCPMC4117357
OpenAlexW2027283911

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.