ArticleJournal of oral science2014
MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro.
Article in Journal of oral science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 29 citations in OpenAlex.
- Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis-A Systematic Review.Biomolecules · 2022Pooled it
- The Role of microRNAs in Pulp Inflammation.Cells · 2021Pooled it
- Article
- MicroRNAs Function in Dental Stem Cells as a Promising Biomarker and Therapeutic Target for Dental Diseases.Molecular diagnosis & therapy · 2023Review
- MicroRNAs and Periodontal Disease: Helpful Therapeutic Targets?Advanced pharmaceutical bulletin · 2023Review
- TNFα, IL-6, miR-103a-3p, miR-423-5p, miR-23a-3p, miR-15a-5p and miR-223-3p in the crevicular fluid of periodontopathic patients correlate with each other and at different stages of the disease.Scientific reports · 2023Article
- MicroRNA-155 expression is associated with pulpitis progression by targeting SHIP1.Molecular biology reports · 2022Article
- Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review.International journal of molecular sciences · 2022Review
- Emerging role of epigenetic regulations in periodontitis: a literature review.American journal of translational research · 2022Review
- Expression of miR-155 and miR-146a in the saliva of patients with periodontitis and its clinical value.American journal of translational research · 2021Article
- Biosensor and Lab-on-a-chip Biomarker-identifying Technologies for Oral and Periodontal Diseases.Frontiers in pharmacology · 2020Review
- Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis.Journal of cellular and molecular medicine · 2019Article
- Role of Osterix and MicroRNAs in Bone Formation and Tooth Development.Medical science monitor : international medical journal of experimental and clinical research · 2016Review
- MicroRNAs as Salivary Markers for Periodontal Diseases: A New Diagnostic Approach?BioMed research international · 2016Review
- The negative feedback regulation of microRNA-146a in human periodontal ligament cells after Porphyromonas gingivalis lipopolysaccharide stimulation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs showing a tissue-specific expression pattern, and whose function is to suppress protein synthesis. In this study, we hypothesized that expression of miRNAs would differ among fibroblasts from dental pulp (DPF), gingiva (GF) and periodontal ligament (PLF) in vitro. Once established by an explant technique, DPF, GF and PLF were collected for RNA isolation and subjected to a miRNA microarray. Next, cells were stimulated with E. coli lipopolysaccharide (LPS) for 24 h and then collected for RNA isolation. Expression of miR-146a and miR-155 was investigated by qPCR. Microarray screening revealed several miRNAs that showed specifically high expression in at least one of the fibroblast subtypes. These molecules are potentially involved in the regulation of extracellular matrix turnover and production of inflammatory mediators. Microarray analysis showed that both miR-146a and miR-155 were among the miRNAs expressed exclusively in GF. qPCR demonstrated significant upregulation of miR-146a only in GF after LPS stimulation, whereas basal expression of miR-155 was higher in GF than in the other cell subtypes. LPS downregulated the expression of miR-155 only in GF. Our results suggest that the expression and regulation of miR-146a and miR-155 are more pronounced in GF than in DPF and PLF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.