ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2014
Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- The diagnosis of microorganism involved in infective endocarditis (IE) by polymerase chain reaction (PCR) and real-time PCR: A systematic review.The Kaohsiung journal of medical sciences · 2018Pooled it
- Prolonged Culture Negativity in Infective Endocarditis Following Short-Term Antibiotic Exposure: A Case Report.Cureus · 2025Article
- Advances and Prospects in the Diagnosis and Treatment of Blood Culture-Negative Infective Endocarditis.Reviews in cardiovascular medicine · 2025Review
- From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis.Pathogens (Basel, Switzerland) · 2025Review
- The Evolving Landscape of Infective Endocarditis: Difficult-to-Treat Resistance Bacteria and Novel Diagnostics at the Foreground.Journal of clinical medicine · 2025Review
- Intraoperative Polymerase Chain Reaction from Cardiac Valve Tissue Is Beneficial for Guiding Further Therapy in Patients with Infective Endocarditis.Journal of clinical medicine · 2024Article
- Novel Diagnostic Methods for Infective Endocarditis.International journal of molecular sciences · 2024Review
- Universal PCR for bacteria, mycobacteria, and fungi: a 10-year retrospective review of clinical indications and patient outcomes.Journal of clinical microbiology · 2023Article
- Updates in Culture-Negative Endocarditis.Pathogens (Basel, Switzerland) · 2023Review
- Targeting the 16S rRNA Gene by Reverse Complement PCR Next-Generation Sequencing: Specific and Sensitive Detection and Identification of Microbes Directly in Clinical Samples.Microbiology spectrum · 2023Article
- Molecular and Serological Diagnostic Approach to Define the Microbiological Origin of Blood Culture-Negative Infective Endocarditis.Pathogens (Basel, Switzerland) · 2022Article
- 16S rDNA PCR for the aetiological diagnosis of culture-negative infective endocarditis.Infection · 2022Article
- Article
- Blood culture negative infective endocarditis in adult congenital heart disease patients with prosthetic grafts: a case series.European heart journal. Case reports · 2021Article
- Article
- Observational
- The role of real-time PCR testing in the investigation of paediatric patients with community-onset osteomyelitis and septic arthritis.Irish journal of medical science · 2019Article
- Pathology of the Aortic Valve: Aortic Valve Stenosis/Aortic Regurgitation.Current cardiology reports · 2019Review
- Experimental procedures for decontamination and microbiological testing in cardiovascular tissue banks.Experimental biology and medicine (Maywood, N.J.) · 2018Review
- Molecular Diagnosis of Bacterial Definite Infective Endocarditis by Real-Time Polymerase Chain Reaction.Cardiology research · 2018Article
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Authors and funding
9 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infective endocarditis (IE) can be diagnosed in the clinical microbiology laboratory by culturing explanted heart valve material. We present a service evaluation that examines the sensitivity and specificity of a broad-range 16S rDNA polymerase chain reaction (PCR) assay for the detection of the causative microbe in culture-proven and culture-negative cases of IE. A clinical case-note review was performed for 151 patients, from eight UK and Ireland hospitals, whose endocardial specimens were referred to the Microbiology Laboratory at Great Ormond Street Hospital (GOSH) for broad-range 16S rDNA PCR over a 12-year period. PCR detects the causative microbe in 35/47 cases of culture-proven IE and provides an aetiological agent in 43/69 cases of culture-negative IE. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the 16S rDNA PCR assay were calculated for this series of selected samples using the clinical diagnosis of IE as the reference standard. The values obtained are as follows: sensitivity = 67 %, specificity = 91 %, PPV = 96 % and NPV = 46 %. A wide range of organisms are detected by PCR, with Streptococcus spp. detected most frequently and a relatively large number of cases of Bartonella spp. and Tropheryma whipplei IE. PCR testing of explanted heart valves is recommended in addition to culture techniques to increase diagnostic yield. The data describing the aetiological agents in a large UK and Ireland series of culture-negative IE will allow future development of the diagnostic algorithm to include real-time PCR assays targeted at specific organisms.
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