ArticleMedical science monitor : international medical journal of experimental and clinical research2014
TESTIN suppresses tumor growth and invasion via manipulating cell cycle progression in endometrial carcinoma.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- The value of lung function assessment and Testin expression detection in clinicopathological features and prognosis of NSCLC patients.Journal of cardiothoracic surgery · 2024Article
- Study on the expression of testin in the testes of dogs.Journal of veterinary research · 2023Article
- The Expression of Testin, Ki-67 and p16 in Cervical Cancer Diagnostics.Current issues in molecular biology · 2023Article
- Minimally Invasive Surgery for Cervical Cancer in Light of the LACC Trial: What Have We Learned?Current oncology (Toronto, Ont.) · 2022Review
- Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA.Molecular biology of the cell · 2021Article
- Preliminary Study on the Expression of Testin, p16 and Ki-67 in the Cervical Intraepithelial Neoplasia.Biomedicines · 2021Article
- Silencing of Testin expression is a frequent event in spontaneous lymphomas from Trp53-mutant mice.Scientific reports · 2020Article
- A comparative analysis of gene expression induced by the embryo in the caprine endometrium.Veterinary medicine and science · 2020Article
- The Role of Testin in Human Cancers.Pathology oncology research : POR · 2019Review
- TES functions as a Mena-dependent tumor suppressor in gastric cancer carcinogenesis and metastasis.Cancer communications (London, England) · 2019Article
- Testin protects against cardiac hypertrophy by targeting a calcineurin-dependent signalling pathway.Journal of cellular and molecular medicine · 2019Article
- The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions.PloS one · 2017Article
- TESTIN Induces Rapid Death and Suppresses Proliferation in Childhood B Acute Lymphoblastic Leukaemia Cells.PloS one · 2016Article
- Effects of a simulated CO2 pneumoperitoneum environment on the proliferation, apoptosis, and metastasis of cervical cancer cells in vitro.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe TESTIN gene was demonstrated to be a tumor suppressor in prostate and breast cancer through inhibiting tumor growth and invasion. Herein, we aimed to investigate the detailed functions of TESTIN in the highly sexual hormone (estrogen)-dependent malignancy, endometrial carcinoma. MATERIAL AND
methodsTESTIN mRNA and protein expression were measured by qRT-PCR, Western blot and immunohistochemistry. Upregulation of TESTIN was achieved by transfecting the pcDNA3.1-TESTIN plasmids into AN3CA cells. Knockdown of TESTIN was achieved by transfecting the shRNA-TESTIN into Ishikawa cells. MTT assay, colony formation assay, and Transwell assay were used to investigate the effects of TESTIN on cellular proliferation and invasion. The apoptotic status and cell cycle were analyzed using flow cytometry. MMP2 secretion was determined by ELISA assay. The xenograft assay was used to investigate the functions of TESTIN in nude mice.
resultsCompared to the non-malignant adjacent endometrium, 54% of tumor samples presented downregulation of TESTIN (P<0.001). Loss of TESTIN protein was correlated with advanced tumor stage (P=0.047), high grade (P=0.034), and lymphatic vascular space invasion (P=0.036). In vitro, overexpression of TESTIN suppressed cell proliferation, induced dramatic G1 arrest, and inhibited tumor invasion through blocking the secretion of MMP2. Loss of TESTIN accelerated cellular proliferation, promoted cell cycle progression, and enhanced tumor invasion by increasing the secretion of MMP2. Consistently, TESTIN could significantly delay the growth of xenografts in nude mice.
conclusionsTESTIN was commonly downregulated in human endometrial carcinoma and was associated with poor prognostic markers. Moreover, TESTIN significantly inhibited tumor growth and invasion via arresting cell cycle in in vitro and in vivo experiments. Therefore, we propose that TESTIN might be a prognostic marker and therapeutic target for endometrial carcinoma.
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