ArticleJournal of cell science2014
Polarizing intestinal epithelial cells electrically through Ror2.
Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Spatial transcriptomics defines IM-crypt as a premalignant niche with bidirectional divergence in early gastric carcinogenesis.Communications biology · 2026Article
- Distinct Structural Responses of Lipid Bilayers to Horizontal and Vertical Electric Fields.The journal of physical chemistry letters · 2025Article
- The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway.Current issues in molecular biology · 2024Article
- Inducing Vertebrate Limb Regeneration: A Review of Past Advances and Future Outlook.Cold Spring Harbor perspectives in biology · 2022Review
- Quantifying the transport of biologics across intestinal barrier models in real-time by fluorescent imaging.Frontiers in bioengineering and biotechnology · 2022Article
- Epithelial Physiology, Ovarian Follicles, Nerve Growth Cones, Vibrating Probes, Wound Healing, and Cluster Headache: Staggering Steps on a Route Map to Bioelectricity.Bioelectricity · 2020Article
- Polarized retinal pigment epithelium generates electrical signals that diminish with age and regulate retinal pathology.Journal of cellular and molecular medicine · 2018Article
- Endogenous bioelectric currents promote differentiation of the mammalian lens.Journal of cellular physiology · 2018Article
- The bioelectric code: An ancient computational medium for dynamic control of growth and form.Bio Systems · 2018Review
- Wingless ligand 5a is a critical regulator of placental growth and survival.Scientific reports · 2016Article
- ERK5 signalling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation.Nature communications · 2016Article
- Biofield Physiology: A Framework for an Emerging Discipline.Global advances in health and medicine · 2015Review
- Physiological extracellular electrical signals guide and orient the polarity of gut epithelial cells.Tissue barriersArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical membrane faces the lumen is not well understood. The electrical signal across the epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Here, we show that applying a physiological electric field to intestinal epithelial cells, to mimic the natural electric field created by the transepithelial potential difference, polarized phosphorylation of the actin-binding protein ezrin, increased expression of intestinal alkaline phosphatase (ALPI, a differentiation marker) and remodeled the actin cytoskeleton selectively on the cathode side. In addition, an applied electric field also activated ERK1/2 and LKB1 (also known as STK11), key molecules in apical membrane formation. Disruption of the tyrosine protein kinase transmembrane receptor Ror2 suppressed activation of ERK1/2 and LKB1 significantly, and subsequently inhibited apical membrane formation in enterocytes. Our findings indicate that the endogenous electric field created by the transepithelial potential difference might act as an essential coordinating signal for apical membrane formation at a tissue level, through activation of LKB1 mediated by Ror2-ERK signaling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.