ArticleApplied and environmental microbiology2014
Alternative sigma factors SigF, SigE, and SigG are essential for sporulation in Clostridium botulinum ATCC 3502.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2023Review
- Expression profiles of sporulation genes in multidrug-resistantExperimental biology and medicine (Maywood, N.J.) · 2023Article
- Review
- Sporulation Strategies and Potential Role of the Exosporium in Survival and Persistence ofInternational journal of molecular sciences · 2022Article
- CRISPR-Cas9-Based Toolkit forFrontiers in microbiology · 2021Article
- Effects of Spo0A on Clostridium acetobutylicum with an emphasis on biofilm formation.World journal of microbiology & biotechnology · 2020Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Optimization of alkaline protease production by rational deletion of sporulation related genes in Bacillus licheniformis.Microbial cell factories · 2019Article
- Article
- Sigma Factor Regulated Cellular Response in a Non-solvent ProducingFrontiers in microbiology · 2017Article
- Transcriptional analysis of degenerate strain Clostridium beijerinckii DG-8052 reveals a pleiotropic response to CaCOScientific reports · 2016Article
- Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes.Current opinion in microbiology · 2015Review
- The Clostridium sporulation programs: diversity and preservation of endospore differentiation.Microbiology and molecular biology reviews : MMBR · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium botulinum produces heat-resistant endospores that may germinate and outgrow into neurotoxic cultures in foods. Sporulation is regulated by the transcription factor Spo0A and the alternative sigma factors SigF, SigE, SigG, and SigK in most spore formers studied to date. We constructed mutants of sigF, sigE, and sigG in C. botulinum ATCC 3502 and used quantitative reverse transcriptase PCR and electron microscopy to assess their expression of the sporulation pathway on transcriptional and morphological levels. In all three mutants the expression of spo0A was disrupted. The sigF and sigE mutants failed to induce sigG and sigK beyond exponential-phase levels and halted sporulation during asymmetric cell division. In the sigG mutant, peak transcription of sigE was delayed and sigK levels remained lower than that in the parent strain. The sigG mutant forespore was engulfed by the mother cell and possessed a spore coat but no peptidoglycan cortex. The findings suggest that SigF and SigE of C. botulinum ATCC 3502 are essential for early sporulation and late-stage induction of sigK, whereas SigG is essential for spore cortex formation but not for coat formation, as opposed to previous observations in B. subtilis sigG mutants. Our findings add to a growing body of evidence that regulation of sporulation in C. botulinum ATCC 3502, and among the clostridia, differs from the B. subtilis model.
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