ArticleApplied and environmental microbiology2014
Metaproteomic identification of diazotrophic methanotrophs and their localization in root tissues of field-grown rice plants.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- From Traditional to Omics-Driven: Emerging Strategies for Isolation, Cultivation, and Identification of Plant Endophytes.Plants (Basel, Switzerland) · 2026Review
- Recent Advances and Developments in Bacterial Endophyte Identification and Application: A 20-Year Landscape Review.Plants (Basel, Switzerland) · 2025Review
- Variations in the NRice (New York, N.Y.) · 2025Article
- Unelongated Stems are an Active Nitrogen-Fixing Site in Rice Stems Supported by Both Sugar and Methane Under Low Nitrogen Conditions.Rice (New York, N.Y.) · 2025Article
- High strain-level diversity of Bradyrhizobium across Australian soils.The ISME journal · 2025Article
- Expanding the Eco-collection of Methane-oxidizing Bacteria Inhabiting Rice Roots: Cultivation, Isolation, and Genomic Characterization of Isolates.Microbes and environments · 2025Article
- Low microbial diversity, yeast prevalence, and nematode-trapping fungal presence in fungal colonization and leaf microbiome of Serjania erecta.Scientific reports · 2024Article
- A Complete Metaproteomic Workflow for Arabidopsis Roots Inoculated by Synthetic Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Microbiota and functional analyses of nitrogen-fixing bacteria in root-knot nematode parasitism of plants.Microbiome · 2023Article
- The Promises, Challenges, and Opportunities of Omics for Studying the Plant Holobiont.Microorganisms · 2022Article
- Prospecting the significance of methane-utilizing bacteria in agriculture.World journal of microbiology & biotechnology · 2022Review
- Article
- Ensuring future food security and resource sustainability: insights into the rhizosphere.iScience · 2022Review
- Mitigation of Paddy Field Soil Methane Emissions by Betaproteobacterium Azoarcus Inoculation of Rice Seeds.Microbes and environments · 2022Article
- Influence of host genotype in establishing root associated microbiome ofFrontiers in microbiology · 2022Article
- Methanotrophs Contribute to Nitrogen Fixation in Emergent Macrophytes.Frontiers in microbiology · 2022Article
- High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Frontiers in plant science · 2022Article
- Nitrogen fixation in maize: breeding opportunities.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Soil Biogeochemical Cycle Couplings Inferred from a Function-Taxon Network.Research (Washington, D.C.) · 2021Article
- Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content.Frontiers in plant science · 2021Article
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8 authors.
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Abstract
In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsible for CH4 oxidation and N2 fixation under the field conditions. Metaproteomic analysis of root-associated bacteria from field-grown rice (Oryza sativa Nipponbare) revealed that nitrogenase complex-containing nitrogenase reductase (NifH) and the alpha subunit (NifD) and beta subunit (NifK) of dinitrogenase were mainly derived from type II methanotrophic bacteria of the family Methylocystaceae, including Methylosinus spp. Minor nitrogenase proteins such as Methylocella, Bradyrhizobium, Rhodopseudomonas, and Anaeromyxobacter were also detected. Methane monooxygenase proteins (PmoCBA and MmoXYZCBG) were detected in the same bacterial group of the Methylocystaceae. Because these results indicated that Methylocystaceae members mediate both CH4 oxidation and N2 fixation, we examined their localization in rice tissues by using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). The methanotrophs were localized around the epidermal cells and vascular cylinder in the root tissues of the field-grown rice plants. Our metaproteomics and CARD-FISH results suggest that CH4 oxidation and N2 fixation are performed mainly by type II methanotrophs of the Methylocystaceae, including Methylosinus spp., inhabiting the vascular bundles and epidermal cells of rice roots.
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