ArticleParasites & vectors2014
Cultivation of Rickettsia amblyommii in tick cells, prevalence in Florida lone star ticks (Amblyomma americanum).
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- First Molecular Detection ofPathogens (Basel, Switzerland) · 2025Article
- Ticks and Tick-Borne Pathogens in Recreational Greenspaces in North Central Florida, USA.Microorganisms · 2023Article
- Review
- Rickettsia massiliae circulation in sheep and attached Rhipicephalus sanguineus in Central Portugal.Tropical animal health and production · 2022Article
- Brown Dog Tick (International journal of environmental research and public health · 2022Article
- Isolation of known and potentially pathogenic tick-borne microorganisms from European ixodid ticks using tick cell lines.Ticks and tick-borne diseases · 2019Article
- Isolation ofNew microbes and new infections · 2018Article
- The microbiome of neotropical ticks parasitizing on passerine migratory birds.Ticks and tick-borne diseases · 2017Article
- Rickettsia amblyommatis sp. nov., a spotted fever group Rickettsia associated with multiple species of Amblyomma ticks in North, Central and South America.International journal of systematic and evolutionary microbiology · 2016Article
- Rickettsia parkeri Transmission to Amblyomma americanum by Cofeeding with Amblyomma maculatum (Acari: Ixodidae) and Potential for Spillover.Journal of medical entomology · 2015Article
- Vector potential and population dynamics for Amblyomma inornatum.Ticks and tick-borne diseases · 2015Article
- High prevalence of "Candidatus Rickettsia andeanae" and apparent exclusion of Rickettsia parkeri in adult Amblyomma maculatum (Acari: Ixodidae) from Kansas and Oklahoma.Ticks and tick-borne diseases · 2015Article
- Prevalence of Rickettsiales in ticks removed from the skin of outdoor workers in North Carolina.Parasites & vectors · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRickettsia amblyommii is a bacterium in the spotted fever group of organisms associated with the lone star tick (LST), Amblyomma americanum. The LST is the most commonly reported tick to parasitize humans in the southeastern US. Within this geographic region, there have been suspected cases of Rocky Mountain spotted fever (RMSF) where the causative agent, R. rickettsii, was not identified in the local tick population. In these areas, patients with clinical signs of RMSF had low or no detectable antibodies to R. rickettsii, resulting in an inability to confirm a diagnosis.
methodsR. amblyommii was cultivated from host-seeking LSTs trapped in Central Florida and propagated in ISE6 (Ixodes scapularis) and AAE2 (A. americanum) cells. Quantitative PCR targeting the 17-kD gene of Rickettsia spp. identified the genus of the organism in culture. Variable regions of groEL, gtlA and rompA genes were amplified and sequenced to confirm the species. The prevalence of R. amblyommii in LSTs within the geographic region was determined by qPCR followed by conventional PCR and direct sequencing.
resultsAnalyses of amplified sequences from the cultured organism were 100% homologous to R. amblyommii. The overall prevalence of Rickettsia spp. in the local population of LSTs was 57.1% and rompA sequence analysis identified only R. amblyommii in LSTs.
conclusionsA Florida strain of R. amblyommii was successfully cultivated in two tick cell lines. Further evaluation of the new strain and comparisons to the other geographic strains is needed. The prevalence of this SFG organism in the tick population warrants further investigation into the organism's ability to cause clinical disease in mammalian species.
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