ArticleProceedings of the National Academy of Sciences of the United States of America2014
Antibody repertoire deep sequencing reveals antigen-independent selection in maturing B cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Single-cell analysis reveals multi-faceted features of B cell development, together with age-associated B cell subpopulations.Communications biology · 2026Article
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- Contrasting effects of SARS-CoV-2 vaccination vs. infection on antibody and TCR repertoires.PloS one · 2026Article
- Genetic background shapes the naïve IgM B cell receptor repertoire in mice.Frontiers in immunology · 2026Article
- Commensal Bacteria Drive B-cell Lymphomagenesis in the Setting of Innate Immunodeficiency.Blood cancer discovery · 2025Article
- Contrasting Effects of SARS-CoV-2 Vaccination vs. Infection on Antibody and TCR Repertoires.bioRxiv : the preprint server for biology · 2025Article
- B cell receptor repertoire abnormalities in autoimmune disease.Frontiers in immunology · 2024Review
- IGH rod-like tracer: An AlphaFold2 structural similarity extraction-based predictive biomarker for MRD monitoring in pre-B-ALL.iScience · 2023Article
- Repertoire-scale measures of antigen binding.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Deconvolution of B cell receptor repertoire in multiple sclerosis patients revealed a delay in tBreg maturation.Frontiers in immunology · 2022Article
- Human transitional and IgMImmunology and cell biology · 2021Article
- The Hydropathy Index of the HCDR3 Region of the B-Cell Receptor Identifies Two Subgroups of IGHV-Mutated Chronic Lymphocytic Leukemia Patients With Distinct Outcome.Frontiers in oncology · 2021Article
- Article
- Tracing Self-Reactive B Cells in Normal Mice.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- B cells, plasma cells and antibody repertoires in the tumour microenvironment.Nature reviews. Immunology · 2020Review
- Deep Characterization of the Human Antibody Response to Natural Infection Using Longitudinal Immune Repertoire Sequencing.Molecular & cellular proteomics : MCP · 2020Article
- Immune repertoire fingerprinting by principal component analysis reveals shared features in subject groups with common exposures.BMC bioinformatics · 2019Article
- Selection of immunoglobulin elbow region mutations impacts interdomain conformational flexibility in HIV-1 broadly neutralizing antibodies.Nature communications · 2019Article
- Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq.Nature protocols · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibody repertoires are known to be shaped by selection for antigen binding. Unexpectedly, we now show that selection also acts on a non-antigen-binding antibody region: the heavy-chain variable (VH)-encoded "elbow" between variable and constant domains. By sequencing 2.8 million recombined heavy-chain genes from immature and mature B-cell subsets in mice, we demonstrate a striking gradient in VH gene use as pre-B cells mature into follicular and then into marginal zone B cells. Cells whose antibodies use VH genes that encode a more flexible elbow are more likely to mature. This effect is distinct from, and exceeds in magnitude, previously described maturation-associated changes in heavy-chain complementarity determining region 3, a key antigen-binding region, which arise from junctional diversity rather than differential VH gene use. Thus, deep sequencing reveals a previously unidentified mode of B-cell selection.
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