ArticleAnalytical chemistry2014
Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Lossless Altered Histone Modification Analysis System (LAHMAS).Lab on a chip · 2025Article
- Extraction of redox extracellular vesicles using exclusion-based sample preparation.Analytical and bioanalytical chemistry · 2024Article
- Improving wastewater-based epidemiology performance through streamlined automation.Journal of environmental chemical engineering · 2023Article
- Longitudinal Molecular Profiling of Circulating Tumor Cells in Metastatic Renal Cell Carcinoma.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2022Article
- Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2.Nature communications · 2021Article
- Centrifugation-Assisted Immiscible Fluid Filtration for Dual-Bioanalyte Extraction.Analytical chemistry · 2019Article
- Point-of-Care HIV Viral Load Testing: an Essential Tool for a Sustainable Global HIV/AIDS Response.Clinical microbiology reviews · 2019Review
- Versatile exclusion-based sample preparation platform for integrated rare cell isolation and analyte extraction.Lab on a chip · 2018Article
- A bench-top automated workstation for nucleic acid isolation from clinical sample types.Journal of microbiological methods · 2018Article
- Article
- RNA-mediated TILDA for improved cell capacity and enhanced detection of multiply-spliced HIV RNA.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Interrogating Bronchoalveolar Lavage Samples via Exclusion-Based Analyte Extraction.SLAS technology · 2017Article
- Integrated Analysis of Multiple Biomarkers from Circulating Tumor Cells Enabled by Exclusion-Based Analyte Isolation.Clinical cancer research : an official journal of the American Association for Cancer Research · 2017Article
- Magnetic System for Automated Manipulation of Paramagnetic Particles.Analytical chemistry · 2016Article
- AirJump: Using Interfaces to Instantly Perform Simultaneous Extractions.ACS applied materials & interfaces · 2016Article
- A two-magnet strategy for improved mixing and capture from biofluids.Biomicrofluidics · 2016Article
- High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1.PloS one · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Sample preparation is a major bottleneck in many biological processes. Paramagnetic particles (PMPs) are a ubiquitous method for isolating analytes of interest from biological samples and are used for their ability to thoroughly sample a solution and be easily collected with a magnet. There are three main methods by which PMPs are used for sample preparation: (1) removal of fluid from the analyte-bound PMPs, (2) removal of analyte-bound PMPs from the solution, and (3) removal of the substrate (with immobilized analyte-bound PMPs). In this paper, we explore the third and least studied method for PMP-based sample preparation using a platform termed Sliding Lid for Immobilized Droplet Extractions (SLIDE). SLIDE leverages principles of surface tension and patterned hydrophobicity to create a simple-to-operate platform for sample isolation (cells, DNA, RNA, protein) and preparation (cell staining) without the need for time-intensive wash steps, use of immiscible fluids, or precise pinning geometries. Compared to other standard isolation protocols using PMPs, SLIDE is able to perform rapid sample preparation with low (0.6%) carryover of contaminants from the original sample. The natural recirculation occurring within the pinned droplets of SLIDE make possible the performance of multistep cell staining protocols within the SLIDE by simply resting the lid over the various sample droplets. SLIDE demonstrates a simple easy to use platform for sample preparation on a range of complex biological samples.
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