ArticlePloS one2014
ROP18 is a key factor responsible for virulence difference between Toxoplasma gondii and Neospora caninum.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- A candidate virulence factor of Eimeria tenella (EtROP30) predicted by virulence enhancement of transgenic Toxoplasma gondii.Parasitology research · 2023Article
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- Biotinylation of the Neospora caninum parasitophorous vacuole reveals novel dense granule proteins.Parasites & vectors · 2021Article
- Function of Neospora caninum dense granule protein 7 in innate immunity in mice.Parasitology research · 2021Article
- From Signaling Pathways to Distinct Immune Responses: Key Factors for Establishing or CombatingPathogens (Basel, Switzerland) · 2020Review
- The Host Autophagy DuringFrontiers in microbiology · 2020Review
- Identification and characterization of stearoyl-CoA desaturase in Toxoplasma gondii.Acta biochimica et biophysica Sinica · 2019Article
- Genome Wide Identification of Mutational Hotspots in the Apicomplexan Parasite Neospora caninum and the Implications for Virulence.Genome biology and evolution · 2018Article
- Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.Parasites & vectors · 2017Article
- Toxoplasma gondii and Neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites.Parasitology research · 2017Article
- New molecular tools in Neospora caninum for studying apicomplexan parasite proteins.Scientific reports · 2017Article
- Article
- Neospora caninum Activates p38 MAPK as an Evasion Mechanism against Innate Immunity.Frontiers in microbiology · 2016Article
- T. gondii rhoptry protein ROP18 induces apoptosis of neural cells via endoplasmic reticulum stress pathway.Parasites & vectors · 2015Article
- Neospora caninum Recruits Host Cell Structures to Its Parasitophorous Vacuole and Salvages Lipids from Organelles.Eukaryotic cell · 2015Article
- Secreted effectors in Toxoplasma gondii and related species: determinants of host range and pathogenesis?Parasite immunology · 2015Review
- Toxoplasma gondii Suppresses Th2-Induced byIranian journal of parasitologyArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum) are both obligate intracellular protozoan parasites and share many common morphological and biological features. Despite these similarities the two parasites differ dramatically in virulence in mice, but the factors involved in virulence differences between the two parasites remain unknown. A secreted serine-threonine kinase called rhoptry protein 18 (ROP18) was identified to play a crucial role on virulence differences among different T. gondii clonal lineages. Intriguingly, we found that ROP18 in Nc1 strain of N. caninum (NcROP18) is a pseudogene due to several interrupting stop codons in the sequence in our previous studies. We assume that the difference of ROP18 leads to virulence difference between T. gondii and N. caninum. We constructed a transgenic N. caninum Nc1 stain by transfecting the TgROP18 from the T. gondii RH strain. Phenotype and virulence assays showed that the expression of TgROP18 in N. caninum did not affect the motility and cell invasion, but resulted in a significant increase in intracellular parasite proliferation and virulence in mice. Immunity-Related GTPase (IRG) phosphorylation assay showed that the transgenic parasite Nc1-TgROP18 was able to phosphorylate IRGs as T. gondii did. The present study indicated that the ROP18 plays a crucial role in virulence of the closely related parasites T. gondii and N. caninum and it is indeed a key factor responsible for the virulence difference between T. gondii and N. caninum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.