ArticleBiomicrofluidics2014
Single cell kinase signaling assay using pinched flow coupled droplet microfluidics.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Editorial: Multiomics advances: bridging gaps in understanding intricate biological systems.Frontiers in plant science · 2026Article
- Emerging Trends in Microfluidic Biomaterials: From Functional Design to Applications.Journal of functional biomaterials · 2025Review
- Enhancing single-cell encapsulation in droplet microfluidics with fine-tunable on-chip sample enrichment.Microsystems & nanoengineering · 2024Article
- Tracking the stochastic growth of bacterial populations in microfluidic droplets.Physical biology · 2022Article
- Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology.Micromachines · 2019Review
- Counting Proteins in Single Cells with Addressable Droplet Microarrays.Journal of visualized experiments : JoVE · 2018Article
- Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis.Molecules (Basel, Switzerland) · 2016Review
- Microfluidics in systems biology-hype or truly useful?Current opinion in biotechnology · 2016Review
- Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.Sensors (Basel, Switzerland) · 2016Review
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection.Microarrays (Basel, Switzerland) · 2015Review
- Melting analysis on microbeads in rapid temperature-gradient inside microchannels for single nucleotide polymorphisms detection.Biomicrofluidics · 2014Article
- Real-time measurement of thrombin generation using continuous droplet microfluidics.Biomicrofluidics · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Droplet-based microfluidics has shown potential in high throughput single cell assays by encapsulating individual cells in water-in-oil emulsions. Ordering cells in a micro-channel is necessary to encapsulate individual cells into droplets further enhancing the assay efficiency. This is typically limited due to the difficulty of preparing high-density cell solutions and maintaining them without cell aggregation in long channels (>5 cm). In this study, we developed a short pinched flow channel (5 mm) to separate cell aggregates and to form a uniform cell distribution in a droplet-generating platform that encapsulated single cells with >55% encapsulation efficiency beating Poisson encapsulation statistics. Using this platform and commercially available Sox substrates (8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline), we have demonstrated a high throughput dynamic single cell signaling assay to measure the activity of receptor tyrosine kinases (RTKs) in lung cancer cells triggered by cell surface ligand binding. The phosphorylation of the substrates resulted in fluorescent emission, showing a sigmoidal increase over a 12 h period. The result exhibited a heterogeneous signaling rate in individual cells and showed various levels of drug resistance when treated with the tyrosine kinase inhibitor, gefitinib.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.