Evidence map›Paper›PMID 24925526›Full record

Trial reportJournal of vascular research2014

Endothelium-derived hyperpolarizing factor mediates bradykinin-stimulated tissue plasminogen activator release in humans.

Ayaz M Rahman, Jonathan R Murrow, Muhiddin A Ozkor, Nino Kavtaradze, Ji Lin, Christine De Staercke, W Craig Hooper, Amita Manatunga, Salim Hayek, Arshed A Quyyumi

Open access · bronzeAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Journal of vascular research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
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  4. Article
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  9. Article
  10. The role of epoxyeicosatrienoic acids in the cardiovascular system.British journal of clinical pharmacology · 2015
    Review
  11. Fibrinolytic Changes in Women with Preeclampsia.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Ayaz M RahmanDivision of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Ga., USA.
Jonathan R Murrow
Muhiddin A Ozkor
Nino Kavtaradze
Ji Lin
Christine De Staercke
W Craig Hooper
Amita Manatunga
Salim Hayek
Arshed A Quyyumi
Emory University · US

Funding

CTSA INFRASTRUCTURE FOR PEDIATRIC RESEARCHUL1RR025008 · NCRR · EMORY UNIVERSITY · PI STEPHENS, DAVID S · 2007 to 2011
$29.0M
Atlanta Clinical and Translational Science Institute (ACTSI) RenewalUL1TR000454 · NCATS · EMORY UNIVERSITY · PI STEPHENS, DAVID S · 2012 to 2016
$25.8M
X LINKED NYSTAGMUS SYNDROME WITH FEATURES OF ALBINISMM01RR000039 · NCRR · EMORY UNIVERSITY · PI VACCARINO, VIOLA · 1985 to 2007
$21.3M
Endothelial Hyperpolarization in HumansR01HL079115 · NHLBI · EMORY UNIVERSITY · PI QUYYUMI, ARSHED A · 2005 to 2008
$1.5M
NCATS NIH HHS UL1 TR000454NCRR NIH HHS M01 RR000039NCRR NIH HHS M01 RR00039NCRR NIH HHS UL1 RR025008NHLBI NIH HHS R01 HL079115NHLBI NIH HHS R01 HL79115
6 · The paper itself

Abstract

aimsBradykinin (BK) stimulates tissue plasminogen activator (t-PA) release from human endothelium. Although BK stimulates both nitric oxide and endothelium-derived hyperpolarizing factor (EDHF) release, the role of EDHF in t-PA release remains unexplored. This study sought to determine the mechanisms of BK-stimulated t-PA release in the forearm vasculature of healthy human subjects.

methodsIn 33 healthy subjects (age 40.3 ± 1.9 years), forearm blood flow (FBF) and t-PA release were measured at rest and after intra-arterial infusions of BK (400 ng/min) and sodium nitroprusside (3.2 mg/min). Measurements were repeated after intra-arterial infusion of tetraethylammonium chloride (TEA; 1 µmol/min), fluconazole (0.4 µmol·min(-1)·l(-1)), and N(G)-monomethyl-L-arginine (L-NMMA, 8 µmol/min) to block nitric oxide, and their combination in separate studies.

resultsBK significantly increased net t-PA release across the forearm (p < 0.0001). Fluconazole attenuated both BK-mediated vasodilation (-23.3 ± 2.7% FBF, p < 0.0001) and t-PA release (from 50.9 ± 9.0 to 21.3 ± 8.9 ng/min/100 ml, p = 0.02). TEA attenuated FBF (-14.7 ± 3.2%, p = 0.002) and abolished BK-stimulated t-PA release (from 22.9 ± 5.7 to -0.8 ± 3.6 ng/min/100 ml, p = 0.0002). L-NMMA attenuated FBF (p < 0.0001), but did not inhibit BK-induced t-PA release (nonsignificant).

conclusionBK-stimulated t-PA release is partly due to cytochrome P450-derived epoxides and is inhibited by K(+)Ca channel blockade. Thus, BK stimulates both EDHF-dependent vasodilation and t-PA release.

Indexed as

AdultBiological FactorsBradykininCytochrome P-450 Enzyme SystemFemaleFluconazoleForearmHumansMaleMiddle AgedNitroprussideomega-N-MethylargininePotassium Channels, Calcium-ActivatedRegional Blood FlowTetraethylammoniumTissue Plasminogen ActivatorBiological FactorsBradykininCytochrome P-450 Enzyme Systemendothelium-dependent hyperpolarization factorFluconazoleNitroprussideomega-N-MethylargininePotassium Channels, Calcium-ActivatedTetraethylammoniumTissue Plasminogen Activator

Identifiers

PMID24925526
PMCPMC4149839
OpenAlexW2041636952

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.