ArticleeLife2014
Single-molecule tracking in live cells reveals distinct target-search strategies of transcription factors in the nucleus.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers.
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Who cites it
201 citing papers in PubMed, 362 citations in OpenAlex.
- Cohesin prevents local mixing of condensed euchromatic domains in living human cells.Nature genetics · 2026Article
- Extracting anomalous diffusion parameters from multi-state ensembles of short single molecule trajectories.bioRxiv : the preprint server for biology · 2026Article
- Graded versus ON/OFF control in quantitative gene expression and epigenetic memory.The EMBO journal · 2026Review
- BLeaching In-cell Single-molecule burstS (BLISS) reveals a small dynamic fraction of HP1α clusters in undifferentiated embryonic stem cells.bioRxiv : the preprint server for biology · 2026Article
- DeepTRACE brings flexible machine learning to single-molecule track analysis.Communications biology · 2026Article
- Cohesin prevents local mixing of condensed euchromatic domains in living human cells.bioRxiv : the preprint server for biology · 2026Article
- QuantiTrack: A unified software to study protein dynamics in living cells.bioRxiv : the preprint server for biology · 2026Article
- Global maps of transcription factor properties reveal threshold-based formation of DNA-bound and mobile clusters.Science advances · 2026Article
- Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Emerging roles of transcriptional condensates as temporal signal integrators.Nature reviews. Genetics · 2025Review
- Single-molecule tracking reveals the dynamic turnover of Ipl1 at the kinetochores inLife science alliance · 2025Article
- Are complex traits underpinned by polygenic molecular traits? A reflection on the complexity of gene expression.Plant & cell physiology · 2025Review
- Cohesin organizes 3D DNA contacts surrounding active enhancers inGenome research · 2025Article
- Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3' processing.The EMBO journal · 2025Article
- Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells.Science advances · 2025Article
- Enhancing fluorescence correlation spectroscopy with machine learning to infer anomalous molecular motion.Biophysical journal · 2025Article
- Transcription regulation by biomolecular condensates.Nature reviews. Molecular cell biology · 2025Review
- Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells.Nature methods · 2025Article
- Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis.Nature communications · 2025Article
- Protein target search diffusion-association/dissociation free energy landscape around DNA binding site with flanking sequences.Biophysical journal · 2025Article
141 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene regulation relies on transcription factors (TFs) exploring the nucleus searching their targets. So far, most studies have focused on how fast TFs diffuse, underestimating the role of nuclear architecture. We implemented a single-molecule tracking assay to determine TFs dynamics. We found that c-Myc is a global explorer of the nucleus. In contrast, the positive transcription elongation factor P-TEFb is a local explorer that oversamples its environment. Consequently, each c-Myc molecule is equally available for all nuclear sites while P-TEFb reaches its targets in a position-dependent manner. Our observations are consistent with a model in which the exploration geometry of TFs is restrained by their interactions with nuclear structures and not by exclusion. The geometry-controlled kinetics of TFs target-search illustrates the influence of nuclear architecture on gene regulation, and has strong implications on how proteins react in the nucleus and how their function can be regulated in space and time.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.