Evidence map›Paper›PMID 24925133›Full record

ArticleJournal of virological methods2014

New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification.

Yonglin Mu, Jiawei Zeng, Qianqian Chen, Jia Liu, Lili Wang, Fujia Yao, Meng Cui, Zhixiang He, Chiyu Zhang, Ming Xiao and 1 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Yonglin MuCollege of Life and Environmental Science, Shanghai Normal University, Shanghai, China; Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Jiawei ZengDujiangyan Medical Center, Dujiangyan City, Sichuan, China.
Qianqian ChenPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Jia LiuPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Lili WangPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Fujia YaoPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Meng CuiPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Zhixiang HePathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Chiyu ZhangPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China.
Ming XiaoCollege of Life and Environmental Science, Shanghai Normal University, Shanghai, China.
Ke LanPathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China. Electronic address: lanke@sibs.ac.cn.
Institut Pasteur of Shanghai · CNPeople's Hospital of Shiyan · CNShanghai Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human respiratory syncytial virus (HRSV) is a seasonal respiratory pathogen that causes respiratory infection in children and the elderly. A new, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid (within 1h), simultaneous detection of A and B group HRSV. Primers specific for groups A and B were designed to amplify the N and L genes of HRSV, respectively. A fluorescent dye, calcein, was used as an indicator for the endpoint visual detection and/or real-time amplification of HRSV RNA. The detection limit of the new method was 281.17 50% tissue culture infective doses (TCID50)/ml for HRSV A and 1.58 TCID50/ml for HRSV B. To evaluate the validity of this method, a comparison with RT-PCR was performed using 77 nasopharyngeal swabs as samples. Both RT-LAMP and RT-PCR detected HRSV in 38 HRSV samples, yielding a positive rate of 49%. Of the RT-LAMP positive samples, 36 (95%) were also positive by RT-PCR, while two were negative by RT-PCR. Among the 36 RT-LAMP and RT-PCR positive samples, 11 belonged to HRSV group A, while 25 belonged to group B. The results show that the new RT-LAMP is simple, rapid and well suited for HRSV diagnosis, especially in a limited-resource setting.

Indexed as

ChildChild, PreschoolDNA PrimersFluoresceinsHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRespiratory Syncytial Virus, HumanRespiratory Syncytial Virus InfectionsReverse TranscriptionSensitivity and SpecificityStaining and LabelingTime FactorsDNA PrimersFluoresceinsfluorexonField testHuman respiratory syncytial virusMolecular diagnosisRT-LAMPVisual detection

Identifiers

PMID24925133
PMCPMC7113655
OpenAlexW1971679625

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.