ArticlePloS one2014
Live pups from evaporatively dried mouse sperm stored at ambient temperature for up to 2 years.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Drying parameters and aging modulate protective properties of vitrified trehalose.bioRxiv : the preprint server for biology · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.BBA advances · 2026Article
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development.The Journal of reproduction and development · 2022Article
- Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.Journal of assisted reproduction and genetics · 2022Article
- Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.Science advances · 2021Article
- Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature.The Journal of reproduction and development · 2019Article
- Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196 °C to 150 °C.Scientific reports · 2019Article
- Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum.Scientific reports · 2018Article
- Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dry Preservation of Spermatozoa: Considerations for Different Species.Biopreservation and biobanking · 2017Review
- Dimethyl sulfoxide and quercetin prolong the survival, motility, and fertility of cold-stored mouse sperm for 10 days.Biology of reproduction · 2017Article
- Effects of Water on Structure and Dynamics of Trehalose Glasses at Low Water Contents and its Relationship to Preservation Outcomes.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples stored at 4°C for 3, 6, 12, 18, and 24 months, the blastocyst formation rate was 61.5%, 49.1%, 31.5%, 32.2%, and 41.4%, respectively. The blastocyst rate for those stored at ambient temperature (∼22°C) for 3, 6, 12, and 18 months was 57.8%, 36.2%, 33.6%, and 34.4%, respectively. Fifteen, eight and three live pups were produced from sperm stored at room temperature for 12, 18, and 24 months, respectively. This is the first report of live offspring produced from dried mouse sperm stored at ambient temperature for up to 2 years. Based on these results, we suggest that evaporative drying is a potentially useful method for the routine preservation of mouse sperm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.