ArticleBMC plant biology2014
Proteomic analysis of proteins related to rice grain chalkiness using iTRAQ and a novel comparison system based on a notched-belly mutant with white-belly.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Decoding heat-induced chalkiness in rice: molecular mechanisms, genetic networks, and mitigation strategies for climate resilience.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- The mysterious dialogue between the embryo, endosperm, and seed coat, and its implications for seed traits.Journal of experimental botany · 2026Review
- Characterization of a Rice GH5_11 Gene Associated with Endosperm and Seed Traits.Plants (Basel, Switzerland) · 2025Article
- Article
- Molecular Mechanisms of Grain Chalkiness Variation in Rice Panicles.Plants (Basel, Switzerland) · 2025Article
- An Integrative Analysis of the Transcriptome and Proteome of Rice Grain Chalkiness Formation Under High Temperature.Plants (Basel, Switzerland) · 2024Article
- Exploring the impact of key physicochemical properties of rice on taste quality and instant rice processing.Frontiers in plant science · 2024Article
- Genome-Wide Analysis and Functional Characterization of Pyruvate Kinase (PK) Gene Family Modulating Rice Yield and Quality.International journal of molecular sciences · 2022Article
- Three Diverse Granule Preparation Methods for Proteomic Analysis of Mature Rice (Molecules (Basel, Switzerland) · 2022Article
- Comparative Metabolite Profiling of Wheat Cultivars (Metabolites · 2022Article
- Response of rice (Oryza sativa L.) cultivars to elevated ozone stress.Environmental monitoring and assessment · 2021Article
- Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice.International journal of molecular sciences · 2021Review
- Metabolic Disturbance Induced by the Embryo Contributes to the Formation of Chalky Endosperm of a Notched-Belly Rice Mutant.Frontiers in plant science · 2021Article
- Identification and Pleiotropic Effect Analysis ofFrontiers in plant science · 2021Article
- Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants.International journal of molecular sciences · 2020Review
- Proteomic profiling reveals differentially expressed proteins associated with amylose accumulation during rice grain filling.BMC genomics · 2020Article
- A Rice Autophagy GeneFrontiers in plant science · 2020Article
- Embryo-Endosperm Interaction and Its Agronomic Relevance to Rice Quality.Frontiers in plant science · 2020Review
- Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics.Biomolecules · 2019Article
- Transcriptome and Proteome-Based Network Analysis Reveals a Model of Gene Activation in Wheat Resistance to Stripe Rust.International journal of molecular sciences · 2019Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrain chalkiness is a complex trait adversely affecting appearance and milling quality, and therefore has been one of principal targets for rice improvement. Eliminating chalkiness from rice has been a daunting task due to the complex interaction between genotype and environment and the lack of molecular markers. In addition, the molecular mechanisms underlying grain chalkiness formation are still imperfectly understood.
resultsWe identified a notched-belly mutant (DY1102) with high percentage of white-belly, which only occurs in the bottom part proximal to the embryo. Using this mutant, a novel comparison system that can minimize the effect of genetic background and growing environment was developed. An iTRAQ-based comparative display of the proteins between the bottom chalky part and the upper translucent part of grains of DY1102 was performed. A total of 113 proteins responsible for chalkiness formation was identified. Among them, 70 proteins are up-regulated and 43 down-regulated. Approximately half of these differentially expressed proteins involved in central metabolic or regulatory pathways including carbohydrate metabolism (especially cell wall synthesis) and protein synthesis, folding and degradation, providing proteomic confirmation of the notion that chalkiness formation involves diverse but delicately regulated pathways. Protein metabolism was the most abundant category, accounting for 27.4% of the total differentially expressed proteins. In addition, down regulation of PDIL 2-3 and BiP was detected in the chalky tissue, indicating the important role of protein metabolism in grain chalkiness formation.
conclusionsUsing this novel comparison system, our comprehensive survey of endosperm proteomics in the notched-belly mutant provides a valuable proteomic resource for the characterization of pathways contributing to chalkiness formation at molecular and biochemical levels.
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