ArticleAngewandte Chemie (International ed. in English)2014
Direct sensing of 5-methylcytosine by polymerase chain reaction.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Advances and challenges in non-canonical nucleic acids data storage.Nature communications · 2026Review
- Quantitative detection of CpG methylation level on G-quadruplex and i-motif-forming DNA by recombinase polymerase amplification.Analytical and bioanalytical chemistry · 2022Article
- Engineering Polymerases for New Functions.Trends in biotechnology · 2019Review
- Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library.Scientific reports · 2019Article
- Fingerprints of Modified RNA Bases from Deep Sequencing Profiles.Journal of the American Chemical Society · 2017Article
- Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity.Scientific reports · 2017Article
- Selective detection of N6-methyladenine in DNAChemical science · 2017Article
- Variants of sequence family B Thermococcus kodakaraensis DNA polymerase with increased mismatch extension selectivity.PloS one · 2017Article
- 5-methylcytosine-sensitive variants of Thermococcus kodakaraensis DNA polymerase.Nucleic acids research · 2016Article
- Direct and site-specific quantification of RNA 2'-O-methylation by PCR with an engineered DNA polymerase.Nucleic acids research · 2016Article
- Modified Nucleotides for Discrimination between Cytosine and the Epigenetic Marker 5-Methylcytosine.Angewandte Chemie (International ed. in English) · 2016Article
- DNA methylation as a potential diagnosis indicator for rapid discrimination of rare cancer cells and normal cells.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The epigenetic control of genes by the methylation of cytosine resulting in 5-methylcytosine (5mC) has fundamental implications for human development and disease. Analysis of alterations in DNA methylation patterns is an emerging tool for cancer diagnostics and prognostics. Here we report that two thermostable DNA polymerases, namely the DNA polymerase KlenTaq derived from Thermus aquaticus and the KOD DNA polymerase from Thermococcus kodakaraensis, are able to extend 3'-mismatched primer strands more efficiently from 5 mC than from unmethylated C. This feature was advanced by generating a DNA polymerase mutant with further improved 5mC/C discrimination properties and its successful application in a novel methylation-specific PCR approach directly from untreated human genomic DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.