ReviewStem cell reviews and reports2014
Pro-chondrogenic effect of miR-221 and slug depletion in human MSCs.
Review in Stem cell reviews and reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Intertwined roles of microRNA-155 and metformin in osteoarthritis: Novel potential diagnostic, prognostic, and therapeutic modulators.World journal of orthopedics · 2025Review
- Loss of the miR-221/222 Cluster Promotes the Pathogenesis of Osteoarthritis in Inflamed Mouse Chondrocytes and Osteoarthritis Models.Inflammation · 2025Article
- A miR-activated hydrogel for the delivery of a pro-chondrogenic microRNA-221 inhibitor as a minimally invasive therapeutic approach for articular cartilage repair.Materials today. Bio · 2025Article
- Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair.Bioactive materials · 2023Review
- Bone marrow mesenchymal stem cells derived exosomal miRNAs can modulate diabetic bone-fat imbalance.Frontiers in endocrinology · 2023Article
- Inhibition of lncRNA RET enhances radio-sensitivity of tumor cells via miR-3179/Slug/PTEN axis.Toxicology research · 2022Article
- Potential of Using Infrapatellar-Fat-Pad-Derived Mesenchymal Stem Cells for Therapy in Degenerative Arthritis: Chondrogenesis, Exosomes, and Transcription Regulation.Biomolecules · 2022Review
- Regulation of microRNA-221, -222, -21 and -27 in articular cartilage subjected to abnormal compressive forces.The Journal of physiology · 2021Article
- Potential of zebrafish as a model to characterise MicroRNA profiles in mechanically mediated joint degeneration.Histochemistry and cell biology · 2020Review
- Molecular regulation of Snai2 in development and disease.Journal of cell science · 2019Review
- Article
- MicroRNA-221 silencing attenuates the degenerated phenotype of intervertebral disc cells.Aging · 2018Article
- Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair.Cellular and molecular life sciences : CMLS · 2017Review
- Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair.Frontiers in bioengineering and biotechnology · 2017Article
- The Potential of microRNAs for Stem Cell-based Therapy for Degenerative Skeletal Diseases.Current molecular biology reports · 2017Review
- MicroRNA-221 is involved in the regulation of osteoporosis through regulates RUNX2 protein expression and osteoblast differentiation.American journal of translational research · 2017Article
- miR-497 inhibits epithelial mesenchymal transition in breast carcinoma by targeting Slug.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Multifaceted signaling regulators of chondrogenesis: Implications in cartilage regeneration and tissue engineering.Genes & diseases · 2015Article
- The Role of miRNAs in Cartilage Homeostasis.Current genomics · 2015Article
- Concurrent expression of Oct4 and Nanog maintains mesenchymal stem-like property of human dental pulp cells.International journal of molecular sciences · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we have inhibited the expression of two negative regulators of chondrogenesis, Slug transcription factor (TF) and the small non-coding single stranded RNA microRNA-221 (miR-221), in human mesenchymal stem cells (MSCs). Our aim was test a new approach to guide the cells toward a chondrocyte - like phenotype, without the employment of differentiating agents, in the prospect of their clinical applications for cell-based cartilage tissue engineering. We have characterized these manipulated cells by gene expression analysis at the RNA and protein levels. We demonstrated that decreased miR-221 or Slug induced an increase of chondrogenic markers, including collagen type II (Col2A1), and the positive chondrogenic TFs Sox9 and TRPS1. Slug and TRPS1 are not direct targets of miR-221 since their expression was not affected by miR-221 content. Further, we showed by gene expression and Chromatin Immunoprecipitation analyses that i. miR-221 is positively regulated by Slug in hMSCs, where Slug and miR-221 high levels hamper cell differentiation, and ii. TRPS1 contributes to maintaining low levels of miR-221, both in hMSCs committed toward chondrogenesis by Slug depletion and in chondrocytes, where the low levels of miR-221 and Slug allow a chondrogenic phenotype.Taken together, our data may be relevant both to understand yet unknown miRNA - TF regulatory loops in cartilage biology and to establish new strategies based on a siRNA approach for cartilage tissue engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.