ArticleCell proliferation2014
BMP4 promotes SSEA-1(+) hUC-MSC differentiation into male germ-like cells in vitro.
Article in Cell proliferation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- Mesenchymal stromal/stem cells and their exosomes for restoration of spermatogenesis in non-obstructive azoospermia: a systemic review.Stem cell research & therapy · 2021Pooled it
- Mesenchymal Stem Cells (MSCs) Therapy for Recovery of Fertility: a Systematic Review.Stem cell reviews and reports · 2018Pooled it
- Enhancing Spermatogenesis in Non-obstructive Azoospermia Through Mesenchymal Stem Cell Therapy22.Current stem cell research & therapy · 2024Review
- Multipotent fetal stem cells in reproductive biology research.Stem cell research & therapy · 2023Review
- Single-cell RNA Sequencing Reveals Thoracolumbar Vertebra Heterogeneity and Rib-genesis in Pigs.Genomics, proteomics & bioinformatics · 2021Article
- Genetic and Genomic Analyses of Service Sire Effect on Female Reproductive Traits in Holstein Cattle.Frontiers in genetics · 2021Article
- Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction.Stem cell research & therapy · 2019Article
- CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling.Scientific reports · 2017Article
- In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells.Stem cell research & therapy · 2017Article
- EIF2S3Y suppresses the pluripotency state and promotes the proliferation of mouse embryonic stem cells.Oncotarget · 2016Article
- Overexpression of CD61 promotes hUC-MSC differentiation into male germ-like cells.Cell proliferation · 2016Article
- Umbilical Cord as Prospective Source for Mesenchymal Stem Cell-Based Therapy.Stem cells international · 2016Review
- Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis.In vitro cellular & developmental biology. Animal · 2016Article
- Beneficial effects of coculturing synovial derived mesenchymal stem cells with meniscus fibrochondrocytes are mediated by fibroblast growth factor 1: increased proliferation and collagen synthesis.Stem cells international · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesRecent studies have demonstrated that primordial germ cells (PGC) can be differentiated from human umbilical cord mesenchymal stem cells (hUC-MSCs), and embryonic stem cells (ESCs) in vitro. Nevertheless, efficiencies were low and unstable. Here, whether hUC-MSCs can be induced to differentiate into germ-like cells with the aid of bone morphogenetic protein (BMP4) was investigated. MATERIALS AND
methodsHuman umbilical cord mesenchymal stem cells were freshly isolated and cultured with BMP4. SSEA-1(+/-) cells were purified using magnetic-activated cell sorting (MACS) from the hUC-MSCs, and further induced with BMP4. Quantitative real-time PCR (qRT-PCR) and immunofluorescence analysis were used to determine PGC and germ-like cell-specific markers.
resultsHuman umbilical cord mesenchymal stem cells differentiated into SSEA-1(+) spherical PGC-like cells efficiently with 12.5 ng/ml BMP4. qRT-PCR and immunofluorescence analysis demonstrated that SSEA-1(+) cells expressed higher levels of PGC-specific markers than SSEA-1(-) cells. Furthermore, SSEA-1(+) cells were induced with BMP4 to differentiate into STRA8, SCP3, DMRT1 and PLZF-positive male germ-like cells, and some sperm-like cells were obtained by 7-14 days after induction.
conclusionThese results suggest that SSEA-1(+) hUC-MSCs can differentiate into male germ-like cells in the presence of BMP4. This study provides an efficient protocol to study germ-cell development using hUC-MSCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.