Evidence map›Paper›PMID 24923630›Full record

ArticleThe Journal of surgical research2014

Fluorescent-tilmanocept for tumor margin analysis in the mouse model.

Ava Hosseini, Jennifer L Baker, Christopher A Tokin, Zhengtao Qin, David J Hall, Dwayne G Stupak, Tomoko Hayashi, Anne M Wallace, David R Vera

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of surgical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Fluorescence-Based Molecular Imaging of Porcine Urinary Bladder Sentinel Lymph Nodes.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2017
    Article
  7. Article
  8. Protein transduction therapy into cochleae via the round window niche in guinea pigs.Molecular therapy. Methods & clinical development · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ava HosseiniDepartment of Surgery, University of San Diego, California.
Jennifer L BakerDepartment of Surgery, University of San Diego, California.
Christopher A TokinDepartment of Surgery, University of San Diego, California.
Zhengtao QinDepartment of Radiology, University of San Diego, California; In Vivo Cancer and Molecular Imaging Center, University of San Diego, California.
David J HallDepartment of Radiology, University of San Diego, California; In Vivo Cancer and Molecular Imaging Center, University of San Diego, California; Moores UCSD Cancer Center, University of San Diego, California.
Dwayne G StupakMoores UCSD Cancer Center, University of San Diego, California; Department of Reproductive Medicine, University of San Diego, California.
Tomoko HayashiMoores UCSD Cancer Center, University of San Diego, California; Department of Reproductive Medicine, University of San Diego, California.
Anne M WallaceDepartment of Surgery, University of San Diego, California; Moores UCSD Cancer Center, University of San Diego, California.
David R VeraDepartment of Radiology, University of San Diego, California; In Vivo Cancer and Molecular Imaging Center, University of San Diego, California; Moores UCSD Cancer Center, University of San Diego, California. Electronic address: dvera@ucsd.edu.
University of San Diego · USImaging Center · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
Targeted Smart Nanoplatforms for Multimode ImagingP50CA128346 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI STUPACK, DWAYNE G. · 2008 to 2012
$6.9M
NCI NIH HHS P30 CA023100NCI NIH HHS P50 CA128346
6 · The paper itself

Abstract

backgroundDendritic cells (DC) are localized in close proximity to cancer cells in many well-known tumors, and thus maybe a useful target for tumor margin assessment. MATERIALS AND

methods[(99m)Tc]- cyanine 7 (Cy7)-tilmanocept was synthesized and in vitro binding assays to bone marrow-derived DC were performed. Fifteen mice, implanted with either 4T1 mouse mammary or K1735 mouse melanoma tumors, were administered 1.0 nmol of [(99m)Tc]-Cy7-tilmanocept via tail vein injection. After fluorescence imaging 1 or 2 h after injection, the tumor, muscle, and blood were assayed for radioactivity to calculate percent-injected dose. Digital images of the tumors after immunohistochemical staining for DC were analyzed to determine DC density.

resultsIn vitro binding demonstrated subnanomolar affinity of [(99m)Tc]-Cy7-tilmanocept to DC (KA = 0.31 ± 0.11 nM). After administration of [(99m)Tc]-Cy7-tilmanocept, fluorescence imaging showed a 5.5-fold increase in tumor signal as compared with preinjection images and a 3.3-fold difference in fluorescence activity when comparing the tumor with the surgical bed after tumor excision. Immunohistochemical staining analysis demonstrated that DC density positively correlated with tumor percent of injected dose per gram (r = 0.672, P = 0.03), and higher DC density was observed at the periphery versus center of the tumor (186 ± 54 K versus 64 ± 16 K arbitrary units, P = 0.001).

conclusions[(99m)Tc]-Cy7-tilmanocept exhibits in vitro and in vivo tumor-specific binding to DC and maybe useful as a tumor margin targeting agent.

Indexed as

BenzothiazolesCarbocyaninesDextransMannansAnimalsCD11c AntigenCell LineCell Line, TumorDendritic CellsFemaleMammary Neoplasms, ExperimentalMelanoma, ExperimentalMiceMice, Inbred BALB CMice, Inbred C3HMicroscopy, FluorescenceBenzothiazolesCarbocyaninesCD11c Antigencyanine dye 7DextransMannanstechnetium-diethylenetriaminepentaacetic acid-mannosyl-dextranTechnetium Tc 99m PentetateDendritic cellsFluorescence imagingLymphoseekTilmanoceptTumor margins

Identifiers

PMID24923630
PMCPMC4201840
OpenAlexW2110615788

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.