ArticleScientific reports2014
Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Stochastic Model of T Cell Repolarization during Target Elimination I.Biophysical journal · 2020Article
- Bidirectional sliding of two parallel microtubules generated by multiple identical motors.Journal of mathematical biology · 2019Article
- Kinesin and Dynein Mechanics: Measurement Methods and Research Applications.Journal of biomechanical engineering · 2018Review
- Automated Multi-Peak Tracking Kymography (AMTraK): A Tool to Quantify Sub-Cellular Dynamics with Sub-Pixel Accuracy.PloS one · 2016Article
- Mapping the global mRNA transcriptome during development of the murine first molar.Frontiers in genetics · 2015Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular cargo is transported by multiple motor proteins. Because of the force balance of motors with mixed polarities, cargo moves bidirectionally to achieve biological functions. Here, we propose a microtubule gliding assay for a tug-of-war study of kinesin and dynein. A boundary of the two motor groups is created by photolithographically patterning gold to selectively attach kinesin to the glass and dynein to the gold surface using a self-assembled monolayer. The relationship between the ratio of two antagonistic motor numbers and the velocity is derived from a force-velocity relationship for each motor to calculate the detachment force and motor backward velocity. Although the tug-of-war involves >100 motors, values are calculated for a single molecule and reflect the collective dynein and non-collective kinesin functions when they work as a team. This assay would be useful for detailed in vitro analysis of intracellular motility, e.g., mitosis, where a large number of motors with mixed polarities are involved.
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