ArticleNature protocols2014
Porous nucleating agents for protein crystallization.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 106 citations in OpenAlex.
- Nucleation of protein crystals in pores and their growth.Biophysical reviews · 2025Review
- Review
- Universality of Hair as a Nucleant: Exploring the Effects of Surface Chemistry and Topography.Crystal growth & design · 2023Article
- Preparation and Characterization of Metal-Organic Framework Coatings for Improving Protein Crystallization Screening.Nanomaterials (Basel, Switzerland) · 2023Article
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality.International journal of molecular sciences · 2022Article
- Article
- Theoretical and experimental investigation of protein crystal nucleation in pores and crevices.IUCrJ · 2021Article
- Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.Journal of applied crystallography · 2019Article
- The Recombinant Inhibitor of DNA Binding Id2 Forms Multimeric Structures via the Helix-Loop-Helix Domain and the Nuclear Export Signal.International journal of molecular sciences · 2018Article
- Langmuir-Blodgett nanotemplates for protein crystallography.Nature protocols · 2017Article
- Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence.Chemical science · 2017Article
- Can the propensity of protein crystallization be increased by using systematic screening with metals?Protein science : a publication of the Protein Society · 2017Review
- Understanding pre-mRNA splicing through crystallography.Methods (San Diego, Calif.) · 2017Review
- Protein crystal nucleation in pores.Scientific reports · 2017Article
- Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments.Scientific reports · 2017Article
- A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield.Scientific reports · 2016Article
- Room Temperature Crystallization of Hydroxyapatite in Porous Silicon Structures.Nanoscale research letters · 2016Article
- Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties.Human vaccines & immunotherapeutics · 2016Article
- Reductively PEGylated carbon nanomaterials and their use to nucleate 3D protein crystals: a comparison of dimensionality.Chemical science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.