ArticleOptics express2014
Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging.
Article in Optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Revealing mitochondrial architecture and functions with single molecule localization microscopy.Biology of the cell · 2025Review
- Interferometric excitation fluorescence lifetime imaging microscopy.Nature communications · 2024Article
- Direct frequency domain fluorescence lifetime imaging using simultaneous ultraviolet and visible excitation.Biomedical optics express · 2023Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Snapshot multicolor fluorescence imaging using double multiplexing of excitation and emission on a single detector.Scientific reports · 2021Article
- Fourier Multiplexed Fluorescence Lifetime Imaging.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Excitation-multiplexed multicolor superresolution imaging with fm-STORM and fm-DNA-PAINT.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Frequency-Encoded Multicolor Fluorescence Imaging with Single-Photon-Counting Color-Blind Detection.Biophysical journal · 2018Article
- Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.Biomedical optics express · 2017Article
- Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging.Nature photonics · 2017Article
- Multiplexed 3D FRET imaging in deep tissue of live embryos.Scientific reports · 2015Article
- FPGA-based multi-channel fluorescence lifetime analysis of Fourier multiplexed frequency-sweeping lifetime imaging.Optics express · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.