ArticleAmerican journal of physiology. Renal physiology2014
Renal blood flow and oxygenation drive nephron progenitor differentiation.
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- 3D printed chip as platform to vascularize hiPSCs-derived kidney organoids.Biomedical microdevices · 2026Article
- Kidney organoid vascularization: current advancements in the field.Regenerative therapy · 2026Article
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- Mitochondrial organization in the developing proximal tubule is controlled by LRRK2.Nature communications · 2025Article
- Early-life exposures and long-term health: adverse gestational environments and the programming of offspring renal and vascular disease.American journal of physiology. Renal physiology · 2024Review
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- RAAS-deficient organoids indicate delayed angiogenesis as a possible cause for autosomal recessive renal tubular dysgenesis.Nature communications · 2023Article
- Vasculogenesis in kidney organoids upon transplantation.NPJ Regenerative medicine · 2022Article
- Vascular deficiencies in renal organoids and ex vivo kidney organogenesis.Developmental biology · 2021Article
- Deletion of hypoxia-responsive microRNA-210 results in a sex-specific decrease in nephron number.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Article
- The novel and critical role of von Hippel-Lindau in nephron formation.Annals of translational medicine · 2019Article
- Von Hippel-Lindau Acts as a Metabolic Switch Controlling Nephron Progenitor Differentiation.Journal of the American Society of Nephrology : JASN · 2019Article
- MAPK/ERK Signaling in Regulation of Renal Differentiation.International journal of molecular sciences · 2019Review
- Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro.BMC biomedical engineering · 2019Article
- Article
- Effect of fluid shear stress onBiomicrofluidics · 2018Article
- Concepts for a therapeutic prolongation of nephrogenesis in preterm and low-birth-weight babies must correspond to structural-functional properties in the nephrogenic zone.Molecular and cellular pediatrics · 2017Review
- Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney.Scientific reports · 2017Article
- Role of hypoxia during nephrogenesis.Pediatric nephrology (Berlin, Germany) · 2016Review
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11 authors at 3 institutions in 1 country.
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Abstract
During kidney development, the vasculature develops via both angiogenesis (branching from major vessels) and vasculogenesis (de novo vessel formation). The formation and perfusion of renal blood vessels are vastly understudied. In the present study, we investigated the regulatory role of renal blood flow and O2 concentration on nephron progenitor differentiation during ontogeny. To elucidate the presence of blood flow, ultrasound-guided intracardiac microinjection was performed, and FITC-tagged tomato lectin was perfused through the embryo. Kidneys were costained for the vasculature, ureteric epithelium, nephron progenitors, and nephron structures. We also analyzed nephron differentiation in normoxia compared with hypoxia. At embryonic day 13.5 (E13.5), the major vascular branches were perfused; however, smaller-caliber peripheral vessels remained unperfused. By E15.5, peripheral vessels started to be perfused as well as glomeruli. While the interior kidney vessels were perfused, the peripheral vessels (nephrogenic zone) remained unperfused. Directly adjacent and internal to the nephrogenic zone, we found differentiated nephron structures surrounded and infiltrated by perfused vessels. Furthermore, we determined that at low O2 concentration, little nephron progenitor differentiation was observed; at higher O2 concentrations, more differentiation of the nephron progenitors was induced. The formation of the developing renal vessels occurs before the onset of blood flow. Furthermore, renal blood flow and oxygenation are critical for nephron progenitor differentiation.
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