Evidence map›Paper›PMID 24920426›Full record

ArticleProteomics. Clinical applications2014

N-glycoprotein surfaceomes of four developmentally distinct mouse cell types.

Erin M Kropp, Subarna Bhattacharya, Matthew Waas, Sandra L Chuppa, Anna-Katerina Hadjantonakis, Kenneth R Boheler, Rebekah L Gundry

Abstract read
In one paragraph

Article in Proteomics. Clinical applications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021
    Review
  3. The in silico human surfaceome.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Erin M KroppDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
Subarna Bhattacharya
Matthew Waas
Sandra L Chuppa
Anna-Katerina Hadjantonakis
Kenneth R Boheler
Rebekah L Gundry

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
Medical Scientist Training ProgramT32GM080202 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI BARBIERI, JOSEPH T, SALZMAN, NITA H · 2010 to 2024
$5.7M
Morphogenesis of the paraxial mesoderm in miceR01HD052115 · NICHD · SLOAN-KETTERING INST CAN RESEARCH · PI HADJANTONAKIS, ANNA-KATERINA · 2006 to 2015
$4.1M
Morphogenesis of mammalian gut endodermR01DK084391 · NIDDK · SLOAN-KETTERING INST CAN RESEARCH · PI HADJANTONAKIS, ANNA-KATERINA · 2009 to 2018
$4.1M
Surface Proteins in the Differentiation of Stem Cells to CardiomyocytesR00HL094708 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI GUNDRY, REBEKAH L. · 2011 to 2013
$702k
NCI NIH HHS P30 CA008748NHLBI NIH HHS 4R00HL094708-03NHLBI NIH HHS R00 HL094708NICHD NIH HHS R01 HD052115NIDDK NIH HHS R01 DK084391NIDDK NIH HHS R01-DK084391NIGMS NIH HHS T32 GM080202
6 · The paper itself

Abstract

purposeDetailed knowledge of cell surface proteins present during early embryonic development remains limited for most cell lineages. Due to the relevance of cell surface proteins in their functional roles controlling cell signaling and their utility as accessible, nongenetic markers for cell identification and sorting, the goal of this study was to provide new information regarding the cell surface proteins present during early mouse embryonic development. EXPERIMENTAL

designUsing the cell surface capture technology, the cell surface N-glycoproteomes of three cell lines and one in vitro differentiated cell type representing distinct cell fates and stages in mouse embryogenesis were assessed.

resultsAltogether, more than 600 cell surface N-glycoproteins were identified represented by >5500 N-glycopeptides. CONCLUSIONS AND CLINICAL RELEVANCE: The development of new, informative cell surface markers for the reliable identification and isolation of functionally defined subsets of cells from early developmental stages will advance the use of stem cell technologies for mechanistic developmental studies, including disease modeling and drug discovery.

Indexed as

Embryonic DevelopmentProteomicsAmino Acid SequenceAnimalsCell LineGlycoproteinsMiceMolecular Sequence DataGlycoproteinsBiomarkersCardiovascularGlycoproteinsGlycoproteomicsPlasma membrane

Identifiers

PMID24920426
PMCPMC4151168

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.