ArticleBMC plant biology2014
The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca(2+) and Mg(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches' Broom Disease: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Defense to3 Biotech · 2025Article
- Priming grapevines with oregano essential oil vapour results in a metabolomic shift eliciting resistance against downy mildew.BMC plant biology · 2024Article
- Decrypting the multi-functional biological activators and inducers of defense responses against biotic stresses in plants.Heliyon · 2023Review
- Full-Length Transcriptome Characterization and Functional Analysis of Pathogenesis-Related Proteins inInternational journal of molecular sciences · 2022Article
- Cloning and Characterization of Two NovelInternational journal of molecular sciences · 2022Article
- Pathogenesis-related protein-4 (PR-4) gene family in Qingke (Hordeum vulgare L. var. nudum): genome-wide identification, structural analysis and expression profile under stresses.Molecular biology reports · 2022Article
- TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.Scientific reports · 2022Article
- Structural and Evolutionary Analyses of PR-4 SUGARWINs Points to a Different Pattern of Protein Function.Frontiers in plant science · 2021Article
- A Pathogenesis-Related Protein-Like Gene Is Involved in theFrontiers in plant science · 2020Article
- CRISPR/Cas9-mediatedHorticulture research · 2020Article
- Plants fight fungi using kiwellin proteins.Nature · 2019Article
- Structural and Functional Characterization of PR-4 SUGARWINs From Sugarcaneand Their Role in Plant Defense.Frontiers in plant science · 2018Article
- Overexpression ofFrontiers in microbiology · 2017Article
- Article
- A Chitin-binding Protein Purified fromFrontiers in microbiology · 2017Article
- Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco.BMC plant biology · 2016Article
- Protein profile and protein interaction network of Moniliophthora perniciosa basidiospores.BMC microbiology · 2016Article
- Theobroma cacao L. pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization.BMC genomics · 2016Article
- The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.Frontiers in plant science · 2016Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b.
resultsTcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection). The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae.
conclusionTo our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively.
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