ArticleJournal of the Royal Society, Interface2014
The role of mechanical forces in the planar-to-bulk transition in growing Escherichia coli microcolonies.
Article in Journal of the Royal Society, Interface, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
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- Growth of nonmotile stress-responsive bacteria in 3D colonies under confining pressure.Biophysical journal · 2025Article
- A review of gut failure as a cause and consequence of critical illness.Critical care (London, England) · 2025Review
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- Interactions between Pseudomonas aeruginosa and six opportunistic pathogens cover a broad spectrum from mutualism to antagonism.Environmental microbiology reports · 2024Article
- The biophysical basis of bacterial colony growth.Nature physics · 2024Article
- Quantifying massively parallel microbial growth with spatially mediated interactions.PLoS computational biology · 2024Article
- Computer simulation study of nutrient-driven bacterial biofilm stratification.Journal of the Royal Society, Interface · 2024Article
- Substrate geometry affects population dynamics in a bacterial biofilm.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multipad agarose plate: a rapid and high-throughput approach for antibiotic susceptibility testing.Journal of the Royal Society, Interface · 2024Article
- Pressure-dependent growth controls 3D architecture of Pseudomonas putida microcolonies.Environmental microbiology reports · 2023Article
- Proliferating active matter.Nature reviews. Physics · 2023Review
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- Active layer dynamics drives a transition to biofilm fingering.NPJ biofilms and microbiomes · 2023Article
- Scattering angle resolved optical coherence tomography measures morphological changes inJournal of biomedical optics · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Mechanical forces are obviously important in the assembly of three-dimensional multicellular structures, but their detailed role is often unclear. We have used growing microcolonies of the bacterium Escherichia coli to investigate the role of mechanical forces in the transition from two-dimensional growth (on the interface between a hard surface and a soft agarose pad) to three-dimensional growth (invasion of the agarose). We measure the position within the colony where the invasion transition happens, the cell density within the colony and the colony size at the transition as functions of the concentration of the agarose. We use a phenomenological theory, combined with individual-based computer simulations, to show how mechanical forces acting between the bacterial cells, and between the bacteria and the surrounding matrix, lead to the complex phenomena observed in our experiments-in particular the observation that agarose concentration non-trivially affects the colony size at transition. Matching these approaches leads to a prediction for how the friction between the bacteria and the agarose should vary with agarose concentration. Our experimental conditions mimic numerous clinical and environmental scenarios in which bacteria invade soft matrices, as well as shedding more general light on the transition between two- and three-dimensional growth in multicellular assemblies.
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