ArticlePloS one2014
Responses of a triple mutant defective in three iron deficiency-induced Basic Helix-Loop-Helix genes of the subgroup Ib(2) to iron deficiency and salicylic acid.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 48 citations in OpenAlex.
- Cellular clarity: a logistic regression approach to identify root epidermal regulators of iron deficiency response.BMC genomics · 2023Article
- Cross-Talk between Iron Deficiency Response and Defense Establishment in Plants.International journal of molecular sciences · 2023Review
- Iron uptake, signaling, and sensing in plants.Plant communications · 2022Review
- Article
- Bacterial CompoundPlants (Basel, Switzerland) · 2020Article
- FIT, a regulatory hub for iron deficiency and stress signaling in roots, and FIT-dependent and -independent gene signatures.Journal of experimental botany · 2020Review
- The iron deficiency response inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Induced Systemic Resistance (ISR) and Fe Deficiency Responses in Dicot Plants.Frontiers in plant science · 2019Review
- The basic helix-loop-helix transcription factor, bHLH11 functions in the iron-uptake system in Arabidopsis thaliana.Journal of plant research · 2019Article
- The Transcriptional Control of Iron Homeostasis in Plants: A Tale of bHLH Transcription Factors?Frontiers in plant science · 2019Article
- Small-Molecules Selectively Modulate Iron-Deficiency Signaling Networks in Arabidopsis.Frontiers in plant science · 2019Article
- FIT-Binding Proteins and Their Functions in the Regulation of Fe Homeostasis.Frontiers in plant science · 2019Review
- Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress.International journal of molecular sciences · 2018Review
- Dissection of iron signaling and iron accumulation by overexpression of subgroup Ib bHLH039 protein.Scientific reports · 2017Article
- Genome-wide analysis of basic helix-loop-helix (bHLH) transcription factors in Brachypodium distachyon.BMC genomics · 2017Article
- Mapping and Characterization of theFrontiers in plant science · 2017Article
- Molecular characterization of the basic helix-loop-helix (bHLH) genes that are differentially expressed and induced by iron deficiency in Populus.Plant cell reports · 2015Article
- Ethylene Participates in the Regulation of Fe Deficiency Responses in Strategy I Plants and in Rice.Frontiers in plant science · 2015Review
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Plants are sessile organisms that adapt to external stress by inducing molecular and physiological responses that serve to better cope with the adverse growth condition. Upon low supply of the micronutrient iron, plants actively increase the acquisition of soil iron into the root and its mobilization from internal stores. The subgroup Ib(2) BHLH genes function as regulators in this response, however their concrete functions are not fully understood. Here, we analyzed a triple loss of function mutant of BHLH39, BHLH100 and BHLH101 (3xbhlh mutant). We found that this mutant did not have any iron uptake phenotype if iron was provided. However, under iron deficiency the mutant displayed a more severe leaf chlorosis than the wild type. Microarray-based transcriptome analysis revealed that this mutant phenotype resulted in the mis-regulation of 198 genes, out of which only 15% were associated with iron deficiency regulation itself. A detailed analysis revealed potential targets of the bHLH transcription factors as well as genes reflecting an exaggerated iron deficiency response phenotype. Since the BHLH genes of this subgroup have been brought into the context of the plant hormone salicylic acid, we investigated whether the 3xbhlh mutant might have been affected by this plant signaling molecule. Although a very high number of genes responded to SA, also in a differential manner between mutant and wild type, we did not find any indication for an association of the BHLH gene functions in SA responses upon iron deficiency. In summary, our study indicates that the bHLH subgroup Ib(2) transcription factors do not only act in iron acquisition into roots but in other aspects of the adaptation to iron deficiency in roots and leaves.
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