ArticleJournal of the American Chemical Society2014
Effects of crowding on the stability of a surface-tethered biopolymer: an experimental study of folding in a highly crowded regime.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed.
- Single-Response Duplexing of Electrochemical Label-Free Biosensor from the Same Tag.Advanced healthcare materials · 2024Article
- Poly-adenine-mediated spherical nucleic acids for interfacial recognition of kanamycin.Mikrochimica acta · 2022Article
- The effect of charged residue substitutions on the thermodynamics of protein-surface interactions.Protein science : a publication of the Protein Society · 2021Article
- Article
- Surface Attachment Enhances the Thermodynamic Stability of Protein L.Angewandte Chemie (International ed. in English) · 2019Article
- Experimental Measurement of Surface Charge Effects on the Stability of a Surface-Bound Biopolymer.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Investigating the dynamics of surface-immobilized DNA nanomachines.Scientific reports · 2016Article
- Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.Chemical science · 2016Article
- Biomolecular Crowding Arising from Small Molecules, Molecular Constraints, Surface Packing, and Nano-Confinement.The journal of physical chemistry letters · 2015Article
- Crowding-induced cooperativity in DNA surface hybridization.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The high packing densities and fixed geometries with which biomolecules can be attached to macroscopic surfaces suggest that crowding effects may be particularly significant under these often densely packed conditions. Exploring this question experimentally, we report here the effects of crowding on the stability of a simple, surface-attached DNA stem-loop. We find that crowding by densely packed, folded biomolecules destabilizes our test-bed biomolecule by ~2 kJ/mol relative to the dilute (noninteracting) regime, an effect that presumably occurs due to steric and electrostatic repulsion arising from compact neighbors. Crowding by a dense brush of unfolded biomolecules, in contrast, enhances its stability by ~6 kJ/mol, presumably due to excluded volume and electrostatic effects that reduce the entropy of the unfolded state. Finally, crowding by like copies of the same biomolecule produces a significantly broader unfolding transition, likely because, under these circumstances, the stabilizing effects of crowding by unfolded molecules increase (and the destabilizing effects of neighboring folded molecules decrease) as more and more neighbors unfold. The crowding of surface-attached biomolecules may thus be a richer, more complex phenomenon than that seen in homogeneous solution.
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Registered trials
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