ArticleSensors (Basel, Switzerland)2014
Application of an electrochemical immunosensor with a MWCNT/PDAA modified electrode for detection of serum trypsin.
Article in Sensors (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 23 citations in OpenAlex.
- Au-decorated NiYFe-LDH/MWCNT nanohybrid-based high-performance immunosensor for ultrasensitive electrochemical detection of cancer antigen 125.Mikrochimica acta · 2026Article
- Nanomaterials functionalized signal-ON/OFF electrogenerated chemiluminescence biosensor for quantization of trypsin based on target-induced cleavage of peptide.Mikrochimica acta · 2025Article
- Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6.Sensors (Basel, Switzerland) · 2020Review
- Fluorescent microsphere probe for rapid qualitative and quantitative detection of trypsin activity.Nanoscale advances · 2019Article
- Label-free nanopore single-molecule measurement of trypsin activity.ACS sensors · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode.
methodA MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
resultsWith optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10-100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL.
conclusionsThe electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.
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