ArticlePloS one2014
Virus enrichment for single virus infection by using 3D insulator based dielectrophoresis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Manipulation, Sampling and Inactivation of the SARS-CoV-2 Virus Using Nonuniform Electric Fields on Micro-Fabricated Platforms: A Review.Micromachines · 2023Review
- A Prominent Cell Manipulation Technique in BioMEMS: Dielectrophoresis.Micromachines · 2020Review
- Determination of the Empirical Electrokinetic Equilibrium Condition of Microorganisms in Microfluidic Devices.Biosensors · 2020Article
- Insulator Based Dielectrophoresis: Micro, Nano, and Molecular Scale Biological Applications.Sensors (Basel, Switzerland) · 2020Review
- Dielectrophoretic Separation of Particles Using Microfluidic Chip with Composite Three-Dimensional Electrode.Micromachines · 2020Article
- Analysis of Bacteriophages with Insulator-Based Dielectrophoresis.Micromachines · 2019Article
- A review of polystyrene bead manipulation by dielectrophoresis.RSC advances · 2019Review
- Assessment of Sub-Micron Particles by Exploiting Charge Differences with Dielectrophoresis.Micromachines · 2017Article
- Dielectrophoresis for Biomedical Sciences Applications: A Review.Sensors (Basel, Switzerland) · 2017Review
- Concentration of Sindbis virus with optimized gradient insulator-based dielectrophoresis.The Analyst · 2016Article
- The Influence of Virus Infection on the Extracellular pH of the Host Cell Detected on Cell Membrane.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed an active virus filter (AVF) that enables virus enrichment for single virus infection, by using insulator-based dielectrophoresis (iDEP). A 3D-constricted flow channel design enabled the production of an iDEP force in the microfluidic chip. iDEP using a chip with multiple active virus filters (AVFs) was more accurate and faster than using a chip with a single AVF, and improved the efficiency of virus trapping. We utilized maskless photolithography to achieve the precise 3D gray-scale exposure required for fabrication of constricted flow channel. Influenza virus (A PR/8) was enriched by a negative DEP force when sinusoidal wave was applied to the electrodes within an amplitude range of 20 Vp-p and a frequency of 10 MHz. AVF-mediated virus enrichment can be repeated simply by turning the current ON or OFF. Furthermore, the negative AVF can inhibit virus adhesion onto the glass substrate. We then trapped and transported one of the enriched viruses by using optical tweezers. This microfluidic chip facilitated the effective transport of a single virus from AVFs towards the cell-containing chamber without crossing an electrode. We successfully transported the virus to the cell chamber (v = 10 µm/s) and brought it infected with a selected single H292 cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.