ArticlePloS one2014
A comparison of the ability of levels of urinary biomarker proteins and exosomal mRNA to predict outcomes after renal transplantation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Extracellular Vesicles in Acute Kidney Injury: Mechanisms, Biomarkers, and Therapeutic Potential.International journal of nanomedicine · 2025Review
- Exosomes as Modulators and Biomarkers of Transplant Immunity.Current transplantation reports · 2023Article
- Extracellular Vesicles: The Future of Diagnosis in Solid Organ Transplantation?International journal of molecular sciences · 2023Review
- Identification of a serum and urine extracellular vesicle signature predicting renal outcome after kidney transplant.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2023Article
- Urinary Extracellular Vesicles in Chronic Kidney Disease: From Bench to Bedside?Diagnostics (Basel, Switzerland) · 2023Review
- Physiopathological role of extracellular vesicles in alloimmunity and kidney transplantation and their use as biomarkers.Frontiers in immunology · 2023Review
- Article
- Extracellular vesicles derived from patients with antibody-mediated rejection induce tubular senescence and endothelial to mesenchymal transition in renal cells.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Article
- Neutrophil gelatinase-associated lipocalin as an immunomodulator in endocrine hypertension.Frontiers in endocrinology · 2022Review
- Proteomic analysis of urinary extracellular vesicles of kidney transplant recipients with BKV viruria and viremia: A pilot study.Frontiers in medicine · 2022Article
- Extracellular Vesicles in Transplantation.Frontiers in immunology · 2022Review
- Review
- Extracellular vesicles in allograft rejection and tolerance.Cellular immunology · 2020Review
- Exosomes in Nephropathies: A Rich Source of Novel Biomarkers.Disease markers · 2020Review
- Early Graft Function in Deceased Donor Renal Recipients: Role of N-Acetylcysteine.Iranian journal of pharmaceutical research : IJPR · 2020Article
- Advances in Detection of Kidney Transplant Injury.Molecular diagnosis & therapy · 2019Review
- Exosomes and microvesicles in normal physiology, pathophysiology, and renal diseases.Pediatric nephrology (Berlin, Germany) · 2019Review
- Molecular profile of urine extracellular vesicles from normo-functional kidneys reveal minimal differences between living and deceased donors.BMC nephrology · 2018Article
- Exploring the RNA landscape of endothelial exosomes.RNA (New York, N.Y.) · 2018Article
- Neutrophil Gelatinase-Associated Lipocalin as a Biomarker of Allograft Function After Renal Transplantation: Evaluation of the Current Status and Future Insights.Artificial organs · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmRNA for biomarkers of kidney injury extracted from urinary exosomes may reflect or predict levels of the corresponding protein after transplantation and clinical outcomes.
methodsUrinary exosomes were isolated from patients following renal transplantation, from healthy controls, and patients with CKD. Expression of exosomal mRNA for the injury biomarkers neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), kidney injury molecule-1 (KIM-1), and cystatin C was compared with the concentrations of corresponding urinary proteins, 18S RNA and serum creatinine.
resultsAll biomarker protein concentrations increased after transplantation, and urinary NGAL and IL-18 at 24 and 168 h correlated with the day 7 creatinine reduction ratio (CRR). Exosomal18S RNA increased after transplantation, but exosomal mRNA for NGAL, IL-18 and cystatin C did not correlate with the day 7 CRR, or urinary biomarker concentrations at any time after transplantation. Exosomal NGAL mRNA was lower 4 h after transplantation than in control exosomes. In contrast, exosomal mRNA for cystatin C was unchanged after transplantation and in CKD, although urinary cystatin C temporarily increased following transplantation. Urinary KIM-1 increased after transplantation, but exosomal mRNA for KIM-1 remained undetectable. In CKD 18S RNA was raised, and exosomal mRNA for NGAL, IL-18 and cystatin C was detected in all patients. While urinary NGAL was greater in CKD than control subjects, exosomal NGAL mRNA was unchanged. Exosomal IL-18 mRNA was increased in CKD, but not IL-18 protein.
conclusionsAfter renal transplantation, urinary NGAL and IL-18 levels reflect the day 7 CRR. However, while mRNA for these biomarkers is present in exosomes, their levels do not reflect or predict urinary biomarker levels or the CRR. This likely reflects the fact that packaging of mRNA in exosomes is selective, and is not necessarily representative of mRNA in the parent cells responsible for biomarker production.
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