Evidence map›Paper›PMID 24918050›Full record

ArticleFEBS open bio2014

Interaction of Sesbania mosaic virus (SeMV) RNA-dependent RNA polymerase (RdRp) with the p10 domain of polyprotein 2a and its implications in SeMV replication.

Kunduri Govind, Arindam Bakshi, Handanahal S Savithri

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.4field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Kunduri GovindDepartment of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Arindam BakshiDepartment of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Handanahal S SavithriDepartment of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Indian Institute of Science Bangalore · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of viral encoded proteins that interact with RNA-dependent RNA polymerase (RdRp) is an important step towards unraveling the mechanism of replication. Sesbania mosaic virus (SeMV) RdRp was shown to interact strongly with p10 domain of polyprotein 2a and moderately with the protease domain. Mutational analysis suggested that the C-terminal disordered domain of RdRp is involved in the interaction with p10. Coexpression of full length RdRp and p10 resulted in formation of RdRp-p10 complex which showed significantly higher polymerase activity than RdRp alone. Interestingly, CΔ43 RdRp also showed a similar increase in activity. Thus, p10 acts as a positive regulator of RdRp by interacting with the C-terminal disordered domain of RdRp.

Indexed as

3AT, 3 amino-1,2,4 triazolCP, coat proteinIPTG, isopropyl-1thio-β-d-galactopyranosideLacZ, β-galactosidaseLB, Luria Bertani brothMEL1, α-galactosidaseMP, movement proteinNi–NTA, nickel–nitrilo tri-acetic acidONPG, ortho-nitrophenyl-β-galactosidePBST, phosphate buffered saline with 0.1% TWEEN 20Pro, proteaseProtein-protein interactionsRdRp, RNA-dependent RNA polymeraseReplicationRNA-dependent RNA polymerase (RdRp)SD, synthetic dropoutSeMV, Sesbania mosaic virusSesbania mosaic virusSobemovirusVPg, viral protein genome linkedY2H, yeast two hybrid

Identifiers

PMID24918050
PMCPMC4050190
OpenAlexW2031450374

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.