Evidence map›Paper›PMID 24918038›Full record

ArticleFEBS open bio2014

Lentiviral vector transduction of spermatozoa as a tool for the study of early development.

Anil Chandrashekran, Ihsan Isa, Jayesh Dudhia, Adrian J Thrasher, Nicholas Dibb, Colin Casimir, Carol Readhead, Robert Winston

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Regulation and roles of Ca2+ stores in human sperm.Reproduction (Cambridge, England) · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anil ChandrashekranDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Ihsan IsaDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Jayesh DudhiaDepartment of Clinical Science Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK.
Adrian J ThrasherMolecular Immunology Unit, UCL-Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK.
Nicholas DibbDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Colin CasimirDepartment of Natural Sciences, School of Science & Technology, Middlesex University, The Burroughs, London NW4 4BT, UK.
Carol ReadheadBiological Imaging Center, California Institute of Technology, Beckman Institute, 139-74, Pasadena, CA 91135, USA.
Robert WinstonDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spermatozoa and lentiviruses are two of nature's most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.

Indexed as

293T, Human embryonic kidney cells7-AAD, 7-Aminoactinomycin DAZT, azidodeoxythimidineCMV, Cytomegalovirus promoterDevelopmentEF-1, Elongation factor 1 alpha promoterGFP, Green Fluorescent ProteinIn vitro fertilisationIVF, in vitro fertilisationLentiviral vectorsLTR, Long Terminal RepeatPGK, Phosphoglycerate kinase promoterSpermatozoaTransductionTransgenicsUCOE, ubiquitous chromatin opening element promoterVSV-g, vesicular stomatitis virus

Identifiers

PMID24918038
PMCPMC4048842

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.