ArticleFrontiers in physiology2014
The buffer capacity of airway epithelial secretions.
Article in Frontiers in physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Molecular Prosthetics and CFTR Modulators Additively Increase Secretory HCOACS chemical biology · 2025Article
- Targeting ATP12A, a Nongastric Proton Pump α Subunit, for Idiopathic Pulmonary Fibrosis Treatment.American journal of respiratory cell and molecular biology · 2023Article
- Review
- Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations.Infectious microbes & diseases · 2022Article
- Heme Peroxidases at Unperturbed and Inflamed Mucous Surfaces.Antioxidants (Basel, Switzerland) · 2021Review
- Large pH oscillations promote host defense against human airways infection.The Journal of experimental medicine · 2021Article
- Is CFTR an exchanger?: Regulation of HCOBiochemistry and biophysics reports · 2021Article
- PHAIR: a biosensor for pH measurement in air-liquid interface cell culture.Scientific reports · 2021Article
- Sulforhodamine B and exogenous surfactant effects on alveolar surface tension under acute respiratory distress syndrome conditions.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Environmental and Endogenous Acids Can Trigger Allergic-Type Airway Reactions.International journal of environmental research and public health · 2020Review
- Measuring pH and Buffer Capacity in Fluids Aspirated from the Fasted Upper Gastrointestinal Tract of Healthy Adults.Pharmaceutical research · 2020Article
- The Contribution of Surface Tension-Dependent Alveolar Septal Stress Concentrations to Ventilation-Induced Lung Injury in the Acute Respiratory Distress Syndrome.Frontiers in physiology · 2020Article
- Pathological mucus and impaired mucus clearance in cystic fibrosis patients result from increased concentration, not altered pH.The European respiratory journal · 2018Article
- Tracheal acid or surfactant instillation raises alveolar surface tension.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- Nominal carbonic anhydrase activity minimizes airway-surface liquid pH changes during breathing.Physiological reports · 2018Article
- Fluid transport by the cornea endothelium is dependent on buffering lactic acid efflux.American journal of physiology. Cell physiology · 2016Article
- Relationships among CFTR expression, HCO3- secretion, and host defense may inform gene- and cell-based cystic fibrosis therapies.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia.The Journal of physiology · 2016Article
- Acidic pH increases airway surface liquid viscosity in cystic fibrosis.The Journal of clinical investigation · 2016Article
- Simulation of Cl(-) Secretion in Epithelial Tissues: New Methodology Estimating Activity of Electro-Neutral Cl(-) Transporter.Frontiers in physiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pH of airway epithelial secretions influences bacterial killing and mucus properties and is reduced by acidic pollutants, gastric reflux, and respiratory diseases such as cystic fibrosis (CF). The effect of acute acid loads depends on buffer capacity, however the buffering of airway secretions has not been well characterized. In this work we develop a method for titrating micro-scale (30 μl) volumes and use it to study fluid secreted by the human airway epithelial cell line Calu-3, a widely used model for submucosal gland serous cells. Microtitration curves revealed that HCO(-) 3 is the major buffer. Peak buffer capacity (β) increased from 17 to 28 mM/pH during forskolin stimulation, and was reduced by >50% in fluid secreted by cystic fibrosis transmembrane conductance regulator (CFTR)-deficient Calu-3 monolayers, confirming an important role of CFTR in HCO(-) 3 secretion. Back-titration with NaOH revealed non-volatile buffer capacity due to proteins synthesized and released by the epithelial cells. Lysozyme and mucin concentrations were too low to buffer Calu-3 fluid significantly, however model titrations of porcine gastric mucins at concentrations near the sol-gel transition suggest that mucins may contribute to the buffer capacity of ASL in vivo. We conclude that CFTR-dependent HCO(-) 3 secretion and epithelially-derived proteins are the predominant buffers in Calu-3 secretions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.