ArticleThe Journal of antimicrobial chemotherapy2014
Mechanisms of reduced susceptibility and genotypic prediction of antibiotic resistance in Prevotella isolated from cystic fibrosis (CF) and non-CF patients.
Article in The Journal of antimicrobial chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Mass azithromycin distribution and antibiotic resistance in the gut and nasopharynx: a cluster-randomized trial.Nature medicine · 2026Trial
- Isolation and identification of hoylesella marshii causing pleural infection: a case report.BMC infectious diseases · 2024Article
- Proteome level analysis of drug-resistantFrontiers in microbiology · 2023Article
- Genomic diversity and antimicrobial resistance ofMicrobial genomics · 2022Article
- Comparing the taxonomic and functional profiles of gut microbiota from three pig breeds by metagenomic sequencing.Frontiers in genetics · 2022Article
- Differences in Fecal Microbiome and Antimicrobial Resistance between Captive and Free-Range Sika Deer under the Same Exposure of Antibiotic Anthelmintics.Microbiology spectrum · 2021Article
- Prevotella diversity, niches and interactions with the human host.Nature reviews. Microbiology · 2021Review
- Article
- Metagenomics of antimicrobial and heavy metal resistance in the cecal microbiome of fattening pigs raised without antibiotics.Applied and environmental microbiology · 2021Article
- Cystic Fibrosis: Recent Insights into Inhaled Antibiotic Treatment and Future Perspectives.Antibiotics (Basel, Switzerland) · 2021Review
- Potential Contributions of Anaerobes in Cystic Fibrosis Airways.Journal of clinical microbiology · 2021Review
- Complex Microbiome in Brain Abscess Revealed by Whole-Genome Culture-Independent and Culture-Based Sequencing.Journal of clinical medicine · 2019Article
- Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum.Journal of bacteriology · 2018Article
- Anaerobic bacteria cultured from cystic fibrosis airways correlate to milder disease: a multisite study.The European respiratory journal · 2018Article
- A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection.Metabolic brain disease · 2018Article
- Article
- Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes.Journal of applied oral science : revista FOB · 2016Article
- Gyrase Mutations Are Associated with Variable Levels of Fluoroquinolone Resistance in Mycobacterium tuberculosis.Journal of clinical microbiology · 2016Article
- Production of extended-spectrum β-lactamases and the potential indirect pathogenic role of Prevotella isolates from the cystic fibrosis respiratory microbiota.International journal of antimicrobial agents · 2016Article
- Multidrug Efflux Systems in Microaerobic and Anaerobic Bacteria.Antibiotics (Basel, Switzerland) · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
objectivesTo investigate mechanisms of reduced susceptibility to commonly used antibiotics in Prevotella cultured from patients with cystic fibrosis (CF), patients with invasive infection and healthy control subjects and to determine whether genotype can be used to predict phenotypic resistance.
methodsThe susceptibility of 157 Prevotella isolates to seven antibiotics was compared, with detection of resistance genes (cfxA-type gene, ermF and tetQ), mutations within the CfxA-type β-lactamase and expression of efflux pumps.
resultsPrevotella isolates positive for a cfxA-type gene had higher MICs of amoxicillin and ceftazidime compared with isolates negative for this gene (P < 0.001). A mutation within the CfxA-type β-lactamase (Y239D) was associated with ceftazidime resistance (P = 0.011). The UK CF isolates were 5.3-fold, 2.7-fold and 5.7-fold more likely to harbour ermF compared with the US CF, UK invasive and UK healthy control isolates, respectively. Higher concentrations of azithromycin (P < 0.001) and clindamycin (P < 0.001) were also required to inhibit the growth of the ermF-positive isolates compared with ermF-negative isolates. Furthermore, tetQ-positive Prevotella isolates had higher MICs of tetracycline (P = 0.001) and doxycycline (P < 0.001) compared with tetQ-negative isolates. Prevotella spp. were also shown, for the first time, to express resistance nodulation division (RND)-type efflux pumps.
conclusionsThis study has demonstrated that Prevotella isolated from various sources harbour a common pool of resistance genes and possess RND-type efflux pumps, which may contribute to tetracycline resistance. The findings indicate that antibiotic resistance is common in Prevotella spp., but the genotypic traits investigated do not reflect phenotypic antibiotic resistance in every instance.
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