ArticleDevelopment (Cambridge, England)2014
Stochastic specification of primordial germ cells from mesoderm precursors in axolotl embryos.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 71 citations in OpenAlex.
- Evolving Lessons on Metazoan Primordial Germ Cells in Diversity and Development.Molecular reproduction and development · 2025Review
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- p38 MAPK as a gatekeeper of reprogramming in mouse migratory primordial germ cells.Frontiers in cell and developmental biology · 2024Article
- Gastruloid-derived primordial germ cell-like cells develop dynamically within integrated tissues.Development (Cambridge, England) · 2023Article
- Identification and Expressional Analysis of Putative PRDI-BF1 and RIZ Homology Domain-Containing Transcription Factors inBiology · 2023Article
- NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl.PLoS biology · 2023Article
- Growth factors, gene activation, and cell recruitment: From intraovarian condensed platelet cytokines toJournal of clinical and translational research · 2022Article
- Primordial Germ Cell Development in the Poeciliid,Frontiers in cell and developmental biology · 2022Article
- Molding immortality from a plastic germline.Current opinion in cell biology · 2021Review
- Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?Biology open · 2021Article
- Residual pluripotency is required for inductive germ cell segregation.EMBO reports · 2021Article
- GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program.Life science alliance · 2021Article
- Does Shoot Apical Meristem Function as the Germline in Safeguarding Against Excess of Mutations?Frontiers in plant science · 2021Review
- Primordial Germ Cell Specification in Vertebrate Embryos: Phylogenetic Distribution and Conserved Molecular Features of Preformation and Induction.Frontiers in cell and developmental biology · 2021Review
- Hedgehog signaling is required for endomesodermal patterning and germ cell development in the sea anemoneeLife · 2020Article
- Mammalian germ cells are determined after PGC colonization of the nascent gonad.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quelling germ cell pluripotency on the genital ridge.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Contrasting patterns of molecular evolution in metazoan germ line genes.BMC evolutionary biology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
A common feature of development in most vertebrate models is the early segregation of the germ line from the soma. For example, in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. How these disparate mechanisms of PGC specification evolved is unknown. Here, in order to identify the ancestral mechanism of PGC specification in vertebrates, we studied PGC specification in embryos from the axolotl (Mexican salamander), a model for the tetrapod ancestor. In the axolotl, PGCs develop within mesoderm, and classic studies have reported their induction from primitive ectoderm (animal cap). We used an axolotl animal cap system to demonstrate that signalling through FGF and BMP4 induces PGCs. The role of FGF was then confirmed in vivo. We also showed PGC induction by Brachyury, in the presence of BMP4. These conditions induced pluripotent mesodermal precursors that give rise to a variety of somatic cell types, in addition to PGCs. Irreversible restriction of the germ line did not occur until the mid-tailbud stage, days after the somatic germ layers are established. Before this, germline potential was maintained by MAP kinase signalling. We propose that this stochastic mechanism of PGC specification, from mesodermal precursors, is conserved in vertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.