ArticleMolecular genetics and genomics : MGG2014
Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique.
Article in Molecular genetics and genomics : MGG, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea.Frontiers in genetics · 2022Pooled it
- ABA-Induced Transcriptomic Dynamics inPlants (Basel, Switzerland) · 2026Article
- Genome-wide DNA methylation and their transgenerational pattern differ in Arabidopsis thaliana populations originated along the elevation of West Himalaya.BMC plant biology · 2024Article
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- Unravelling the Epigenetic Code: DNA Methylation in Plants and Its Role in Stress Response.Epigenomes · 2024Review
- DNA Methylation Dynamics in Response to Drought Stress in Crops.Plants (Basel, Switzerland) · 2024Review
- Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Changes Underlying Adaptation to Low Temperature ofPlants (Basel, Switzerland) · 2024Article
- DNA methylation: an emerging paradigm of gene regulation under drought stress in plants.Molecular biology reports · 2024Review
- Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress.Plants (Basel, Switzerland) · 2023Article
- Exploring the crop epigenome: a comparison of DNA methylation profiling techniques.Frontiers in plant science · 2023Review
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- Epigenetic and Physiological Responses to Varying Root-Zone Temperatures in Greenhouse Rocket.Genes · 2022Article
- Response of Wheat DREB Transcription Factor to Osmotic Stress Based on DNA Methylation.International journal of molecular sciences · 2021Article
- Exploration of Epigenetics for Improvement of Drought and Other Stress Resistance in Crops: A Review.Plants (Basel, Switzerland) · 2021Review
- In Response to Abiotic Stress, DNA Methylation Confers EpiGenetic Changes in Plants.Plants (Basel, Switzerland) · 2021Review
- Transcription FactorFrontiers in plant science · 2019Article
- Epigenetic responses to abiotic stresses during reproductive development in cereals.Plant reproduction · 2018Review
- The influence of AlJournal of applied genetics · 2018Article
- Transgenerational Effects and Epigenetic Memory in the Clonal PlantFrontiers in plant science · 2018Article
- Recurrent water deficit causes epigenetic and hormonal changes in citrus plants.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Perennial ryegrass (Lolium perenne), an excellent grass for forage and turf, is widespread in temperate regions. Drought is an important factor that limits its growth, distribution, and yield. DNA methylation affects gene expression and plays an important role in adaptation to adverse environments. In this study, the DNA methylation changes in perennial ryegrass under drought stress were assessed using methylation-sensitive amplified polymorphism (MSAP). After 15 days of drought stress treatment, the plant height was less than half of the control, and the leaves were smaller and darker. Genome-wide, a total of 652 CCGG sites were detected by MSAP. The total methylation level was 57.67 and 47.39 % in the control and drought treatment, respectively, indicating a decrease of 10.28 % due to drought exposure. Fifteen differentially displayed DNA fragments in MSAP profiles were cloned for sequencing analysis. The results showed that most of the genes involved in stress responses. The relative expression levels revealed that three demethylated fragments were up-regulated. The expression of a predicted retrotransposon increased significantly, changing from hypermethylation to non-methylation. Although the extent of methylation in two other genes decreased, the sites of methylation remained, and the expression increased only slightly. All of these results suggested that drought stress decreased the total DNA methylation level in perennial ryegrass and demethylation up-regulated related gene expressions and that the extent of methylation was negatively correlated with expression. Overall, the induced epigenetic changes in genome probably are an important regulatory mechanism for acclimating perennial ryegrass to drought and possibly other environmental stresses.
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