ArticleBMC microbiology2014
The oxygen sensor MgFnr controls magnetite biomineralization by regulation of denitrification in Magnetospirillum gryphiswaldense.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
- Controlled Low-Oxygen Supply Enables Magnetosome Size Tuning by Uncoupling Magnetite Nucleation and Crystal Growth in Magnetospirillum gryphiswaldense.Microbial biotechnology · 2026Article
- Biosynthesis and function of magnetic organelles in magnetotactic bacteria.Nature reviews. Microbiology · 2026Review
- No evidence for the putative nitric oxide sensor NsrR as a key regulator of magnetosome formation in Magnetospirillum gryphiswaldense.Nucleic acids research · 2026Article
- Influence of a 7 T magnetic field on growth, biomineralization, and denitrification metabolism inApplied and environmental microbiology · 2025Article
- The interplay of carbon and nitrogen cycling driven by watershed microorganisms.Frontiers in microbiology · 2025Review
- Nitric oxide sensor NsrR is the key direct regulator of magnetosome formation and nitrogen metabolism in Magnetospirillum.Nucleic acids research · 2024Article
- The transcriptomic landscape of Magnetospirillum gryphiswaldense during magnetosome biomineralization.BMC genomics · 2022Article
- Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense.mSystems · 2020Article
- An automated oxystat fermentation regime for microoxic cultivation of Magnetospirillum gryphiswaldense.Microbial cell factories · 2020Article
- Repeated horizontal gene transfers triggered parallel evolution of magnetotaxis in two evolutionary divergent lineages of magnetotactic bacteria.The ISME journal · 2020Article
- Magnetic genes: Studying the genetics of biomineralization in magnetotactic bacteria.PLoS genetics · 2020Review
- Novel Protein Mg2046 Regulates Magnetosome Synthesis inFrontiers in microbiology · 2019Article
- Development of a simple intensified fermentation strategy for growth of Magnetospirillum gryphiswaldense MSR-1: Physiological responses to changing environmental conditions.New biotechnology · 2018Article
- High-Throughput Microfluidic Sorting of Live Magnetotactic Bacteria.Applied and environmental microbiology · 2018Article
- The Disruption of an OxyR-Like Protein Impairs Intracellular Magnetite Biomineralization inFrontiers in microbiology · 2017Article
- Magnetosome biogenesis in magnetotactic bacteria.Nature reviews. Microbiology · 2016Review
- A novel role for Crp in controlling magnetosome biosynthesis in Magnetospirillum gryphiswaldense MSR-1.Scientific reports · 2016Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMagnetotactic bacteria are capable of synthesizing magnetosomes only under oxygen-limited conditions. However, the mechanism of the aerobic repression on magnetite biomineralization has remained unknown. In Escherichia coli and other bacteria, Fnr (fumarate and nitrate reduction regulator) proteins are known to be involved in controlling the switch between microaerobic and aerobic metabolism. Here, we report on an Fnr-like protein (MgFnr) and its role in growth metabolism and magnetite biomineralization in the alphaproteobacterium Magnetospirillum gryphiswaldense.
resultsDeletion of Mgfnr not only resulted in decreased N2 production due to reduced N2O reductase activity, but also impaired magnetite biomineralization under microaerobic conditions in the presence of nitrate. Overexpression of MgFnr in the WT also caused the synthesis of smaller magnetite particles under anaerobic and microaerobic conditions in the presence of nitrate. These data suggest that proper expression of MgFnr is required for WT-like magnetosome synthesis, which is regulated by oxygen. Analyses of transcriptional gusA reporter fusions revealed that besides showing similar properties to Fnr proteins reported in other bacteria, MgFnr is involved in the repression of the expression of denitrification genes nor and nosZ under aerobic conditions, possibly owing to several unique amino acid residues specific to MTB-Fnr.
conclusionsWe have identified and thoroughly characterized the first regulatory protein mediating denitrification growth and magnetite biomineralization in response to different oxygen conditions in a magnetotactic bacterium. Our findings reveal that the global oxygen regulator MgFnr is a genuine O2 sensor. It is involved in controlling expression of denitrification genes and thereby plays an indirect role in maintaining proper redox conditions required for magnetite biomineralization.
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